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AFLP reveals structural details of genetic diversity within cultivated olive germplasm from the Eastern Mediterranean

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Abstract

Amplified fragment length polymorphism (AFLP) analysis was used to assess genetic inter-relationships among olive varieties cultivated in the Eastern Mediterranean Basin. The genotypes sampled included most of the important cultivars from Turkey, Greece and the Middle East and selected genotypes from the Western Mediterranean area. A total of 119 polymorphic markers were generated from five selective primer-pair combinations. The combined data sets generated by just two primer-pairs were adequate to discriminate between all 65 genotypes, while each primer-pair could individually identify up to 64 genotypes. A factorial correspondence analysis (FCA) plot indicated that the cultivars clustered into two relatively modestly defined groups. The first broad group was dominated by cultivars from Turkey but also included genotypes originating from the Middle East (Syria and Lebanon) that collectively formed a tight subcluster. The second group comprised Greek cultivars and those originating from the Western Mediterranean. A significant genetic distance value between Greek and Turkish cultivars was provided by an analysis of molecular variance (amova). There was also evidence of substructure here, with an apparent separation of most Spanish and Italian clones. These findings are in general accordance to previous suggestions of an East-West divergence of olive cultivars, although the dichotomy is less extensive than reported previously and complicated by regional variation within each group.

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Acknowledgements

The authors would like to thank Dr. Mike Wilkinson for his helpful suggestions during the preparation of the manuscript. This work was supported by the European Union General Directorate I—Centre International des Haute Etudes Agronomiques Mediterranéennes Joint Initiative ‘Collaborative Mediterranean Biotechnology Network (COMBINE)’.

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Authors and Affiliations

  1. Department of Horticultural Genetics and Biotechnology, Mediterranean Agronomic Institute, Chania, 73100, Greece

    Carolyn A. Owen, Elena-Craita Bita, Shady E. Hajjar, Vardis Sellianakis & Panagiotis Kalaitzis

  2. Department of Horticulture, Faculty of Agriculture, Ege University, Izmir, 35100, Turkey

    Uygun Aksoy & Serra Hepaksoy

  3. Department of Crop Production and Protection, American University of Beirut, Beirut, Lebanon

    Rony Chamoun & Salma N. Talhook

  4. Laboratory of Molecular Biology, Agricultural University of Athens, 11855, Greece

    Georgios Banilas & Polydefkis Hatzopoulos

  5. Institute of Subtropical Plants and Olive Tree, NAGREF, Chania, 73100, Greece

    Ioannis Metzidakis

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  1. Carolyn A. Owen
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  2. Elena-Craita Bita
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  3. Georgios Banilas
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  4. Shady E. Hajjar
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  9. Salma N. Talhook
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  10. Ioannis Metzidakis
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  11. Polydefkis Hatzopoulos
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  12. Panagiotis Kalaitzis
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Correspondence to Panagiotis Kalaitzis.

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Communicated by E. Guiderdoni

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Owen, C.A., Bita, EC., Banilas, G. et al. AFLP reveals structural details of genetic diversity within cultivated olive germplasm from the Eastern Mediterranean. Theor Appl Genet 110, 1169–1176 (2005). https://doi.org/10.1007/s00122-004-1861-z

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  • DOI: https://doi.org/10.1007/s00122-004-1861-z

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