Abstract
Seed pods of wild-type narrow-leafed lupins (Lupinus angustifolius L.) shatter upon maturity, dispersing their seeds. Recessive alleles of the genes Tardus and Lentus that confer reduced pod shattering have been incorporated into domesticated cultivars to facilitate harvesting. Tardus was mapped in an F8 recombinant inbred population of a cross between domesticated and wild lupins. A microsatellite–anchored fragment length polymorphism marker (TaM1), which mapped 2.1 cM from Tardus, was converted to a locus-specific PCR assay. Marker TaM2, a restriction fragment length polymorphism marker was converted to a PCR assay and mapped to 3.9 cM on the other side of Tardus. Marker TaM3, a cleaved amplified polymorphic sequence marker, was positioned along-side marker TaM1 at 3.9 cM from Tardus. One or more markers was polymorphic in 70% of possible pairwise crosses between Australian domesticated lines and wild accessions tested, indicating wide applicability of the markers in crosses between wild and domesticated germplasm.
Similar content being viewed by others
References
Boersma JG, Pallotta M, Li C, Buirchell BJ, Sivasithamparam K, Yang H (2005) Construction of a genetic linkage map using MFLP and identification of molecular markers linked to domestication genes in narrow-leafed lupin (Lupinus angustifolius L.). Cell Mol Biol Lett 10:331–344
Boersma JG, Buirchell BA, Sivasithamparam K, Yang H (2007) Development of two sequence-specific PCR markers linked to the le gene that reduces pod shattering in narrow-leafed Lupin (Lupinus angustifolius L.). Genet Mol Biol 30:623–629. doi:10.1590/S1415-47572007000400020
Cowling WA, Huyghe C, Swiecicki W (1998) Lupin breeding. In: Gladstones JS, Atkins C, Hamblin J (eds) Lupins as crop plants. CAB International University Press, Cambridge, pp 93–120
Dolezel J, Bartos J, Voglmayr H, Greilhuber J (2003) Nuclear DNA content and genome size of trout and human. Cytometry 51:127–128. doi:10.1002/cyto.a.10013
Gladstones JS (1967) Selection for economic characters in Lupinus angustifolius and L. digitatus 1. Non-shattering pods. Aust J Exp Agric Anim Husb 7:360–366. doi:10.1071/EA9670360
Gladstones JS (1998) Distribution, origin, taxonomy, history and importance. In: Gladstones JS, Atkins C, Hamblin J (eds) Lupins as crop plants. CAB International University Press, Cambridge, pp 1–39
Kasprzak A, Šafář J, Janda J, Doležel J, Wolko B, Naganowska B (2006) The bacterial artificial chromosome (BAC) library of the narrow-leafed lupin (Lupinus angustifolius L.). Cell Mol Biol Lett 11:396–407. doi:10.2478/s11658-006-0033-3
Manly KF, Cudmore RH Jr, Meer JM (2001) MapManager QTX, cross-platform software for genetic mapping. Mamm Genome 12:930–932. doi:10.1007/s00335-001-1016-3
Naganowska B, Wolko B, Sliwinska E, Kaczmarek Z (2003) Nuclear DNA content variation and species relationships in the genus Lupinus (Fabaceae). Ann Bot (Lond) 92:349–355. doi:10.1093/aob/mcg145
Nelson MN, Phan HTT, Ellwood SR, Moolhuijzen PM, Bellgard M, Hane J, Williams A, O’Lone CE, Fosu-Nyarko J, Scobie M, Cakir M, Jones MGK, Książkiewicz Wolko B, Barker SJ, Oliver RP, Cowling WA (2006) The first gene-based map of Lupinus angustifolius L.—location of domestication genes and conserved synteny with Medicago truncatula. Theor Appl Genet 113(2):225–238. doi:10.1007/s00122-006-0288-0
Pigeaire A, Abernethy D, Smith PM, Simpson K, Fletcher N, Lu CY, Atkins CA, Cornish E (1997) Transformation of a grain legume (Lupinus angustifolius L.) via Agrobacterium tumefaciens-mediated gene transfer to shoot apices. Mol Breed 3:341–349. doi:10.1023/A:1009642620907
Swiecicki W, Swiecicki WK (1995) Domestication and breeding of narrow-leafed lupin (Lupinus angustifolius L.). J Appl Genet 36:155–167
Voorrips RE (2002) MapChart: Software for the graphical presentation of linkage maps and QTLs. J Hered 93:177–178. doi:10.1093/jhered/93.1.77
Yang H, Sweetingham MW, Cowling WA, Smith PMC (2001) DNA fingerprinting based on micro-satellite anchored fragment length polymorphisms, and isolation of sequence-specific PCR markers in lupin (Lupinus angustifolius L.). Mol Breed 7:203–209. doi:10.1023/A:1011363205557
Yang H, Shankar M, Buirchell BJ, Sweetingham MW, Caminero C, Smith PMC (2002) Development of molecular markers using MFLP linked to a gene conferring resistance to Diaporthe toxica in narrow-leafed lupin (Lupinus angustifolius L.). Theor Appl Genet 105:265–270. doi:10.1007/s00122-002-0925-1
Yang H, Boersma JG, You M, Buirchell BJ, Sweetingham MW (2004) Development and implementation of a sequence-specific PCR marker linked to a gene conferring resistance to anthracnose disease in narrow-leafed lupin (Lupinus angustifolius L.). Mol Breed 14:145–151. doi:10.1023/B:MOLB.0000038003.49638.97
You M, Boersma JG, Buirchell BJ, Sweetingham MW, Siddique KHM, Yang H (2004) A PCR-based molecular marker applicable for marker-assisted selection for anthracnose disease resistance in lupin breeding. Cell Mol Biol Lett 10:123–134
Acknowledgements
The help of Colin Smith (DAFWA) in preliminary phenotyping work and, the advice of Dr John Gladstones on particular aspects of phenotyping, are gratefully acknowledged.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Boersma, J.G., Nelson, M.N., Sivasithamparam, K. et al. Development of sequence-specific PCR markers linked to the Tardus gene that reduces pod shattering in narrow-leafed lupin (Lupinus angustifolius L.). Mol Breeding 23, 259–267 (2009). https://doi.org/10.1007/s11032-008-9230-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11032-008-9230-2