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Mapping quantitative trait loci controlling common bunt resistance in a doubled haploid population derived from the spring wheat cross RL4452 × AC Domain

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Abstract

Wheat resistance to common bunt is a highly desirable trait for environmentally friendly grain grade protection. Valuable breeding achievements have been made to develop wheat varieties with enhanced resistance to the disease, and mapping of race-specific resistance genes has been reported. However, less is known of the chromosomal regions that control non-race specific resistance to common bunt. In this study, we have characterized a segregating population of 185 doubled haploid spring wheat lines derived from the cross RL4452 × AC Domain. Reactions to a mixture of common bunt races were assessed under field simulated spring-sown conditions in greenhouses in two locations over 2 years. A total 369 polymorphic maker loci including 356 microsatellite loci, five expressed sequences tag (ESTs), and eight genes were used to develop a linkage map. Quantitative trait loci (QTL) analysis using composite interval mapping detected three QTLs associated with common bunt resistance, of which two were located on chromosome 1B and one on chromosome 7A. AC Domain alleles contributed the common bunt resistance at all three QTLs. Usefulness of gene tagging within the identified chromosomal regions for common bunt resistance breeding is discussed.

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Acknowledgements

This research was funded by an ARDI Research grant to D. G. Humphreys. This publication is Agriculture and Agri-Food Canada Contribution No. 1954. Authors are grateful to Sheila Woods for statistical advice. We also thank Denis Green and the breeding crew for their technical assistance during bunt disease assessment. We are grateful to Joanne Schiavoni for editing the manuscript and Michael Shillinglaw for preparation of figures.

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Authors and Affiliations

  1. Cereal Research Centre, Agriculture and Agri-Food Canada, 195 Dafoe Rd., Winnipeg, MB, Canada, R3T 2M9

    Bourlaye Fofana, D. Gavin Humphreys, Sylvie Cloutier & Daryl J. Somers

  2. Department of Plant Sciences, College of Agriculture and Bioresources, Agriculture Building, 51 Campus Dr., Saskatoon, SK, Canada, S7N 5A8

    Curt A. McCartney

Authors
  1. Bourlaye Fofana
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  2. D. Gavin Humphreys
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  3. Sylvie Cloutier
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  4. Curt A. McCartney
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  5. Daryl J. Somers
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Correspondence to D. Gavin Humphreys.

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Fofana, B., Humphreys, D.G., Cloutier, S. et al. Mapping quantitative trait loci controlling common bunt resistance in a doubled haploid population derived from the spring wheat cross RL4452 × AC Domain. Mol Breeding 21, 317–325 (2008). https://doi.org/10.1007/s11032-007-9131-9

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  • DOI: https://doi.org/10.1007/s11032-007-9131-9

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