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Comparative mapping of fiber, protein, and mineral content QTLs in two interspecific Leymus wildrye full-sib families

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Abstract

Crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), and mineral content are important components of forage quality in grasses. Elevated [K]/([Ca] + [Mg]) ratios (KRAT) substantially increase the risk of grass tetany (hypomagnesemia) in grazing animals, which is a serious problem associated with some cool-season grasses. The objectives of this study were to map and compare QTLs controlling concentrations of CP, NDF, ADF, Al, B, Ca, Cl, Cu, Fe, K, Mg, Mn, Na, P, S, Si, Zn, and KRAT in two full-sib Leymus triticoides × (L. triticoides × L. cinereus) TTC1 and TTC2 families. Significant genetic variation and QTLs were detected for all traits, with evidence of conserved QTLs for ADF (LG1a, LG5Xm, LG7a), NDF (LG7a), Ca (LG1b), CP, (LG5Xm), KRAT (LG3b, LG6b, LG7a, LG7b), Mn (LG2b, LG3b, LG4Xm), and S (LG3a) content in both TTC1 and TTC2 families. Moreover, the direction of QTL effects was the same for 13 of the 14 homologous QTLs in both families. The TTC1 and TTC2 KRAT QTLs on LG7a and LG7b were located near markers defining homoeologous relationships between the sub-genomes of allotetraploid Leymus, suggesting possible QTL homoeology. Another 88 QTLs were unique to one family or the other, but many of these clustered in genome regions common between the two families. These results will support development of new Leymus wildrye forages and help characterize genes controlling mineral uptake and fiber synthesis.

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Fig. 1

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Abbreviations

ADF:

acid detergent fiber

CP:

crude protein

KRAT:

K/(Ca + Mg) ratio

NDF:

neutral detergent fiber

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Acknowledgements

This work was supported by joint contributions of the USDA Agriculture Research Service, Utah Agriculture Experiment Station, Department of Defense Strategic Environmental Research and Development Program CS1103 project, and US Army BT25-EC-B09 project (Genetic Characterization of Native Plants in Cold Regions). Trade names are included for the benefit of the reader, and imply no endorsement or preferential treatment of the products listed by the USDA.

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Authors and Affiliations

  1. United States Department of Agriculture, Agriculture Research Service, Forage and Range Research Laboratory, Utah State University, Logan, UT, 84322-6300, USA

    Steven R. Larson

  2. Northwest Irrigation and Soils Research Laboratory, Agriculture Research Service, United States Department of Agriculture, Kimberly, ID, 83341, USA

    Henry F. Mayland

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  1. Steven R. Larson
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Correspondence to Steven R. Larson.

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Larson, S.R., Mayland, H.F. Comparative mapping of fiber, protein, and mineral content QTLs in two interspecific Leymus wildrye full-sib families. Mol Breeding 20, 331–347 (2007). https://doi.org/10.1007/s11032-007-9095-9

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