Abstract
Cereal cyst nematode (CCN; Heterodera avenae Woll.) is a root pathogen of cereals that can cause severe yield losses in intolerant wheat cultivars. Loci for resistance to CCN, measured by a seedling bioassay, were identified by creating a genetic map based on a Trident/Molineux doubled haploid population of 182 lines. A novel locus accounting for up to 14% of the resistance to CCN was mapped to chromosome 1B of Molineux by association with microsatellite marker loci Xwmc719 and Xgwm140. This locus acts additively with the previously identified CCN resistance loci identified on chromosomes 6B (Cre8) and 2A (Cre5 on the VPM1 segment) in this population to explain 44% of the genetic variance for this major wheat pathogen.
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Barr AR, Chalmers KJ, Karakousis A, Kretschmer JM, Manning S, Lance RCM, Lewis J, Jefferies SP, Langridge P (1998) RFLP mapping of a new cereal cyst nematode resistance locus in barley. Plant Breed 117:185–187
Bonhomme A, Gale MD, Koebner RMD, Nicolas P, Jahier J, Bernard M (1995) RFLP analysis of an Aegilops ventricosa chromosome that carries a gene conferring resistance to leaf rust (Puccinia recondita) when transferred to hexaploid wheat. Theor Appl Genet 90:1042–1048
Delibes A, Romero D, Aguaded S, Duce A, Mena M, Lopez-Braña I, Andrés MF, Martin-Sanchez JA, Garcia-Olmedo F (1993) Resistance to the cereal cyst nematode (Heterodera-avenae Woll.) transferred from the wild grass Aegilops-ventricosa to hexaploid wheat by a stepping-stone procedure. Theor Appl Genet 87:402–408
Eastwood RF, Lagudah ES, Appels R, Hannah M, Kollmorgen JF (1991) Triticum tauschii, a novel source of resistance to cereal cyst nematode (Heterodera avenae). Aust J Agric Res 42:69–77
Eastwood RF, Lagudah ES, Appels R (1994) A directed search for DNA sequences tightly linked to cereal cyst nematode resistance genes in Triticum tauschii. Genome 37:311–319
Ellis MH, Spielmeyer W, Gale KR, Rebetzke GJ, Richards RA (2002) Perfect markers for the Rht-B1b and Rht-D1b dwarfing genes in wheat. Theor Appl Genet 105:1038–1042
Fisher JM (1982) Problems with the use of resistance in wheat to the Australian pathotype of Heterodera avenae. EPPO Bull 12:417–421
Fisher JM, Rathjen AJ, Dube AJ (1981) Tolerance of commercial cultivars and breeders lines to Heterodera avenae Woll. Aust J Agric Res 32:545–551
Guidet F, Rogowsky P, Taylor C, Song W, Langridge P (1991) Cloning and characterisation of a new rye-specific repeated sequence. Genome 34:81–87
Haley CS, Knott SA (1992) A simple regression method for mapping quantitative trait loci in line crosses using flanking markers. Heredity 69:315–324
Jahier J, Abelard P, Tanguy AM, Dedryver F, Rivoal R, Khatkar S, Bariana HS (2001) The Aegilops ventricosa segment on chromosome 2AS of the wheat cultivar ‘VPM1’ carries the cereal cyst nematode resistance gene Cre5. Plant Breed 120:125–128
Karakousis A, Gustafson JP, Chalmers KJ, Barr AR, Langridge P (2003) A consensus map of barley integrating SSR, RFLP, and AFLP markers. Aust J Agric Res 54:1173–1185
Kretschmer JM, Chalmers KJ, Manning S, Karakousis A, Barr AR, Islam AKMR, Logue SJ, Choe YW, Barker SJ, Lance RCM, Langridge P (1997) RFLP mapping of the Ha2 cereal cyst nematode resistance gene in barley. Theor Appl Genet 94:1060–1064
Kuchel H, Ye G, Fox R, Jefferies S (2005) Genetic and economic analysis of a targeted marker-assisted wheat breeding strategy. Mol Breed 16:67–78
Manly KF, Cudmore RH Jr, Meer JM (2001) Map Manager QTX, cross-platform software for genetic mapping. Mamm Genome 12:930–932
Meagher JW (1977) World dissemination of the cereal nematode (Heterodera avenae) and its potential as a pathogen of wheat. J Nematol 9:9–15
O’Brien PC, Fisher JM (1974) Resistance within wheat, barley and oat cultivars to Heterodera avenae in South Australia. Aust J Exp Agric Anim Husb 14:399–404
Ogbonnaya FC, Seah S, Delibes A, Jahier J, Lopez-Brana I, Eastwood RF, Lagudah ES (2001) Molecular-genetic characterisation of a new nematode resistance gene in wheat. Theor Appl Genet 102:623–629
Paull JG, Chalmers KJ, Karakousis A, Kretschmer JM, Manning S, Langridge P (1998) Genetic diversity in Australian wheat varieties and breeding material based on RFLP data. Theor Appl Genet 96:435–446
Payne RW, Baird DB, Cherry M, Gilmour AR, Harding SA, Kane AK, Lane PW, Murray DA, Soutar DM, Thompson R, Todd AD, Tunnicliffe Wilson G, Webster R, Welham SJ (2002) GenStat release 6.1 reference manual. VSN International, Oxford
Ranjbar GA (1997) Production and utilization of doubled haploid lines in wheat breeding programs. PhD Thesis, University of Adelaide
Rivoal R, Dosba F, Jahier J, Pierre JS (1986) Les lignées d’additionblé Aegilops ventricosa Tausch. VI. Etude de la localisation chromosomique de la résistance à l’égard d’ Heterodera avenae Woll. Agronomie 6:143–148
Romero MD, Montes MJ, Sin E, Lopez-Braña I, Duce A, Martín-Sanchez JA, Andrés MF, Delibes A (1998) A cereal cyst nematode (Heterodera avenae Woll.) resistance gene transferred from Aegilops triuncialis to hexaploid wheat. Theor Appl Genet 96:1135–1140
Rogowsky PM, Guidet FLY, Langridge P, Shepherd KW, Koebner RMD (1991) Isolation and characterization of wheat-rye recombinants involving chromosome arm 1DS of wheat. Theor Appl Genet 82:537–544
Taylor C, Shepherd KW, Langridge P (1998) A molecular genetic map of the long arm of chromosome 6R of rye incorporating the cereal cyst nematode resistance gene, CreR. Theor Appl Genet 97:1000–1012
Williams KJ, Fisher JM, Langridge P (1994) Identification of RFLP markers linked to the cereal cyst nematode resistance gene (Cre) in wheat. Theor Appl Genet 89:927–930
Williams KJ, Lewis JG, Bogacki P, Pallotta M, Willsmore KL, Kuchel H, Wallwork H (2003) Mapping of a QTL contributing to cereal cyst nematode tolerance and resistance in wheat. Aust J Agric Res 54:731–737
Yan L, Helguera M, Kato K, Fukuyama S, Sherman J, Dubcovsky J (2004) Allelic variation at the VRN-1 promotor region in polyploid wheat. Theor Appl Genet 109:1677–1686
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Williams, K.J., Willsmore, K.L., Olson, S. et al. Mapping of a novel QTL for resistance to cereal cyst nematode in wheat. Theor Appl Genet 112, 1480–1486 (2006). https://doi.org/10.1007/s00122-006-0251-0
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DOI: https://doi.org/10.1007/s00122-006-0251-0