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QTL analyses of fiber components and crude protein in an annual × perennial ryegrass interspecific hybrid population

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Abstract

Annual (Lolium multiflorum Lam.) and perennial (Lolium perenne L.) ryegrasses are two important forage and turfgrass species. Improving the digestibility of forage by decreasing fiber content is a major goal in forage crop breeding programs. An annual × perennial ryegrass interspecific hybrid population was used to map quantitative trait loci (QTLs) for fiber components, neutral detergent fiber (NDF), acid detergent fiber (ADF), and acid detergent lignin (ADL), and crude protein (CP). Samples were harvested three times in August and September 2003 and August 2004, respectively. Simple interval mapping was used to detect QTLs from both the male and female parental maps previously developed for the population. Fiber components were all correlated positively with each other and were negatively correlated with CP. The largest correlations were between NDF and ADF with r = 0.86, 0.72, and 0.82 for each of the three harvests. All four traits showed intermediate broad-sense heritability values ranging from 0.35 to 0.72. A total of 63 QTLs were detected for the four traits measured over the three harvests from both the female and male maps. Coincident QTLs were detected on linkage groups (LGs) 2, 6, and 7 for NDF, LGs 1, 2, and 7 for ADF, LGs 6 and 7 for ADL, and LG 2 for CP, respectively. Coincident QTLs were also detected on LGs 2, 6, and 7 for NDF and ADF, providing evidence of the genetic basis of the observed high level of phenotypic correlation. The QTLs on LGs 2, 6, and possibly 7 for fiber components were co-located on the same LG as several lignin biosynthetic genes from perennial ryegrass.

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Abbreviations

ADF:

Acid detergent fiber

ADL:

Acid detergent lignin

CP:

Crude protein

LG:

Linkage group

NDF:

Neutral detergent fiber

QTLs:

Quantitative trait loci

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Acknowledgements

We wish to thank Dr. Mike Casler for critically reading the manuscript. We also express our appreciation to Trish Patrick for technical assistance in laboratory analyses of forage quality traits, and to Lori Evans for technical assistance in maintaining the plants in the greenhouse.

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Authors and Affiliations

  1. Department of Horticulture, Iowa State University, Ames, IA, 50011, USA

    Yanwen Xiong & Shui-zhang Fei

  2. Department of Agronomy, Iowa State University, Ames, IA, 50011, USA

    E. Charles Brummer & Kenneth J. Moore

  3. United States Department of Agriculture-Agriculture Research Service, Corvallis, OR, 97331, USA

    Reed E. Barker

  4. Department of Plant, Soil, and Insect Sciences, University of Massachusetts, Amherst, MA, 01003, USA

    Geunhwa Jung

  5. Department of Plant Pathology, University of Wisconsin, Madison, WI, 53706, USA

    J. Curley

  6. United States Department of Agriculture-Agriculture Research Service, Beltsville, MD, 20705, USA

    Scott E. Warnke

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  1. Yanwen Xiong
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  2. Shui-zhang Fei
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  3. E. Charles Brummer
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  4. Kenneth J. Moore
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  5. Reed E. Barker
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  6. Geunhwa Jung
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  7. J. Curley
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  8. Scott E. Warnke
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Correspondence to Shui-zhang Fei.

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Xiong, Y., Fei, Sz., Brummer, E.C. et al. QTL analyses of fiber components and crude protein in an annual × perennial ryegrass interspecific hybrid population. Mol Breeding 18, 327–340 (2006). https://doi.org/10.1007/s11032-006-9034-1

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  • DOI: https://doi.org/10.1007/s11032-006-9034-1

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