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The Sunflower High-oleic Mutant Ol Carries Variable Tandem Repeats of FAD2-1, a Seed-specific Oleoyl-phosphatidyl Choline Desaturase

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Abstract

Ol, a chemically induced, incompletely dominant mutation, greatly increases oleic acid and is correlated with greatly reduced expression of a seed-specific oleoyl-phosphatidyl choline desaturase (FAD2-1) in developing seeds of sunflower (Helianthus  annuus L.). FAD2-1 is duplicated in high-oleic (mutant) strains and cosegregates with Ol. Codominant RFLP markers have been developed for FAD2-1 and are diagnositic for the Ol mutation; however, the structure of the mutant FAD2-1 locus is unknown and polymorphic sequence-tagged-site (STS) DNA markers have not been developed for FAD2-1. The mutant was discovered to carry tandem repeats of FAD2-1 separated by a 2.67 kb intergenic region. The upstream repeat (FAD2-1U) carries a 1.69 kb intron in the 5′UTR, whereas the downstream repeat (FAD2-1D) is missing the first 1.54 kb of the 5′UTR and intron. Other than the deletion in FAD2-1D, no DNA polymorphisms were identified between wildtype and mutant FAD2-1 alleles among elite oilseed inbred lines. We developed dominant INDEL markers diagnostic for presence or absence of the Ol mutation (tandem FAD2-1 repeats) by targeting DNA sequences upstream of FAD2-1D, identified 49 SNPs and five INDELs (two haplotypes) in DNA sequences downstream of FAD2-1 in the wildtype and FAD2-1U in the mutant, identified polymorphic [AT]n and [GT]n repeats in the 3′UTR of FAD2-1, and developed codominant SSR and INDEL markers for FAD2-1. Novel FAD2-1 alleles found in exotic low-oleic genotypes could be introgressed into elite low-oleic genotypes to facilitate marker-assisted selection of Ol in mid- and high-oleic sunflower breeding programs.

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Abbreviations

FAD2 :

oleoyl-phosphatidyl choline desaturase

FAME:

fatty acid methyl ester

IGR:

intergenic region

FAB2 :

stearoyl-acyl carrier protein desaturase

NJ:

novel joint

LG:

linkage group

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  1. Gunnar F. Schuppert

    Present address: KWS MAIS GMBH, Grimsehlstrasse 31, P.O. Box 1463, 37555, Einbeck, Germany

Authors and Affiliations

  1. Department of Crop and Soil Science, Oregon State University, Corvallis, Oregon, 97331, USA

    Gunnar F. Schuppert & Mary B. Slabaugh

  2. Center for Applied Genetic Technologies, The University of Georgia, 111 Riverbend Road, Athens, Georgia, 30602, USA

    Shunxue Tang & Steven J. Knapp

Authors
  1. Gunnar F. Schuppert
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  2. Shunxue Tang
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  3. Mary B. Slabaugh
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  4. Steven J. Knapp
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Correspondence to Steven J. Knapp.

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Schuppert, G.F., Tang, S., Slabaugh, M.B. et al. The Sunflower High-oleic Mutant Ol Carries Variable Tandem Repeats of FAD2-1, a Seed-specific Oleoyl-phosphatidyl Choline Desaturase. Mol Breeding 17, 241–256 (2006). https://doi.org/10.1007/s11032-005-5680-y

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