Skip to main content
SpringerLink
Log in

Genetic Mapping of the Tph1 Gene Controlling Beta-tocopherol Accumulation in Sunflower Seeds

  • Short Communication
  • Published:
Molecular Breeding Aims and scope Submit manuscript

Abstract

Tocopherols are a family of fat soluble antioxidants of great value for both nutritional and technological properties of seed oils. The four naturally occurring tocopherols (alpha-, beta-, gamma- and delta-tocopherol) widely differ for their relative in vivo (vitamin E) and in vitro antioxidant properties. Sunflower (Helianthus annuus L.) seeds mainly contain alpha-tocopherol (95% of the total tocopherols), which has a great vitamin E value but a low in vitro activity. Conversely, beta-tocopherol shows more balanced in vitro and in vivo antioxidant properties, which is desired for specific uses of the oil. The sunflower line T589 is characterised by an increased beta-tocopherol content in the seeds ( >30%), which is determined by the single gene Tph1. The objectives of this study were to map the Tph1 gene by molecular markers (SSRs) and to develop a linkage map of the Tph1-encompassing region. High performance liquid chromatography (HPLC) was used to phenotype 103 F2 and 67 F3 progeny from the mapping population CAS-12 × T589, which segregates for Tph1. Bulk segregant analysis identified two SSR markers on linkage group (LG) 1 linked to Tph1. A large linkage group was constructed by genotyping additional SSRs and INDEL markers. Tph1 mapped to the upper end of LG 1 and cosegregated with the SSR markers ORS1093, ORS222, and ORS598. The availability of tightly linked PCR-based markers and the location of the Tph1 gene on the sunflower genetic map will be useful for marker-assisted selection in sunflower and provides a basis for the physical mapping and positional cloning of this gene.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • L.S. Conte O. Leoni S. Palmieri P. Capella G. Lercker (1989) ArticleTitleHalf-seed analysis: rapid chromatographic determination of the main fatty acids of sunflower seed Plant Breed. 102 158–165 Occurrence Handle1:CAS:528:DyaL1MXkslCju7k%3D

    CAS  Google Scholar 

  • Z. Cheng S. Sattler H. Maeda Y. Sakuragi D.A. Bryant D. DellaPenna (2003) ArticleTitleHighly divergent methyltransferases catalyze a conserved reaction in tocopherol and plastoquinone synthesis in cyanobacteria and photosynthetic eukaryotes Plant Cell 15 2343–2356 Occurrence Handle1:CAS:528:DC%2BD3sXotlGmtLg%3D Occurrence Handle14508009

    CAS  PubMed  Google Scholar 

  • Y. Demurin (1993) ArticleTitleGenetic variability of tocopherol composition in sunflower seeds Helia 16 59–62

    Google Scholar 

  • Y. Demurin D. Škorić D. Karlovic (1996) ArticleTitleGenetic variability of tocopherol composition in sunflower seeds as a basis of breeding for improved oil quality Plant Breed. 115 33–36 Occurrence Handle1:CAS:528:DyaK28XjtVyrurc%3D

    CAS  Google Scholar 

  • J.M. Fernández-Martínez M. Mancha J. Osorio R. Garcés (1997) ArticleTitleSunflower mutant containing high levels of palmitic acid in high oleic background Euphytica 97 113–116 Occurrence Handle10.1023/A:1003045726610

    Article  Google Scholar 

  • A. Kamal-Eldin L.Å. Appelqvist (1996) ArticleTitleThe chemistry and antioxidant properties of tocopherols and tocotrienols Lipids 31 671–701 Occurrence Handle1:CAS:528:DyaK28XktlKkt74%3D Occurrence Handle8827691

    CAS  PubMed  Google Scholar 

  • E. Lander P. Green J. Abrahanson A. Barlow M. Daley S. Lincoln L. Newburg (1987) ArticleTitleMAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations Genomics 1 174–181 Occurrence Handle10.1016/0888-7543(87)90010-3 Occurrence Handle1:CAS:528:DyaL1cXhsVCksrk%3D Occurrence Handle3692487

    Article  CAS  PubMed  Google Scholar 

  • R.W. Michelmore I. Paran V. Kesseli (1991) ArticleTitleIdentification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations Proc. Natl. Acad. Sci. USA 88 9828–9832 Occurrence Handle1:CAS:528:DyaK38Xhs1emtw%3D%3D Occurrence Handle1682921

    CAS  PubMed  Google Scholar 

  • F.B. Padley F.D. Gunstone J.L. Harwood (1994) Occurrence and characteristics of oils and fats F.D Gunstone J.L. Harwood F.B. Padley (Eds) The Lipid Handbook Chapman & Hall London, United Kingdom 47–223

    Google Scholar 

  • B. Pérez-Vich J.M. Fernández-Martínez M. Grondona S.J. Knapp S.T. Berry (2002) ArticleTitleStearoyl-ACP and oleoyl-PC desaturase genes cosegregate with quantitative trait loci underlying high stearic and high oleic acid mutant phenotypes in sunflower Theor. Appl. Genet. 104 338–349 Occurrence Handle12582706

    PubMed  Google Scholar 

  • B. Pérez-Vich B. Akhtouch S.J. Knapp A.J. Leon L. Velasco J.M. Fernández-Martínez Berry S.T. (2004) ArticleTitleQuantitative trait loci for broomrape (Orobanche cumana Wallr.) resistance in sunflower Theor. Appl. Genet. 109 92–102 Occurrence Handle10.1007/s00122-004-1599-7 Occurrence Handle14968309

    Article  PubMed  Google Scholar 

  • G. Pongracz H. Weiser D. Matzinger (1995) ArticleTitleTocopherole. Antioxidanten der Natur Fat Sci. Technol. 97 90–104 Occurrence Handle1:CAS:528:DyaK2MXkvVCnsLg%3D

    CAS  Google Scholar 

  • B. Savidge J.D. Weiss Y.-H.H. Wong M.W. Lassner T.A. Mitsky C.K. Shewmaker D. Post-Beittenmiller H.E. Valentin (2002) ArticleTitleIsolation and characterization of homogentisate phytyltransferase genes from Synechocystis sp. PCC 6803 and Arabidopsis Plant Physiol. 129 321–332 Occurrence Handle10.1104/pp.010747 Occurrence Handle1:CAS:528:DC%2BD38XjvFSms78%3D Occurrence Handle12011362

    Article  CAS  PubMed  Google Scholar 

  • D. Shintani D. DellaPenna (1998) ArticleTitleElevating the vitamin E content of plants through metabolic engineering Science 282 2098–2100 Occurrence Handle10.1126/science.282.5396.2098 Occurrence Handle1:CAS:528:DyaK1cXotVyisrc%3D Occurrence Handle9851934

    Article  CAS  PubMed  Google Scholar 

  • S. Tang J.K. Yu M. Slabaugh D.K. Shintani S.J. Knapp (2002) ArticleTitleSimple sequence repeat map of the sunflower genome Theor. Appl. Genet. 105 1124–1136 Occurrence Handle1:CAS:528:DC%2BD38XptFKnsr8%3D Occurrence Handle12582890

    CAS  PubMed  Google Scholar 

  • S. Tang V.K. Kishore S.J. Knapp (2003) ArticleTitlePCR-multiplexes for a genome-wide framework of simple sequence repeat marker loci in cultivated sunflower Theor. Appl. Genet. 107 6–19 Occurrence Handle1:CAS:528:DC%2BD3sXkvFyntbs%3D Occurrence Handle12835928

    CAS  PubMed  Google Scholar 

  • L. Velasco J. Domínguez J.M. Fernández-Martínez (2004a) ArticleTitleRegistration of T589 and T2100 sunflower germplasms with modified tocopherol profiles Crop Sci. 44 361–362

    Google Scholar 

  • L. Velasco B. Pérez-Vich J.M. Fernández-Martínez (2004b) ArticleTitleNovel variation for tocopherol profile in sunflower created by mutagenesis and recombination Plant Breed. 123 490–492 Occurrence Handle1:CAS:528:DC%2BD2cXhtVentLjF

    CAS  Google Scholar 

  • L. Velasco B. Pérez-Vich J.M. Fernández-Martínez (2004c) Grain quality in oil crops R. Benech-Arnold R.A. Sánchez (Eds) Handbook of Seed Physiology: Applications to Agriculture Haworth Press Inc. New York, USA 389–427

    Google Scholar 

  • E.M. Vera-Ruiz B. Pérez-Vich J.M. Fernández-Martínez L. Velasco (2005) ArticleTitleComparative genetic study of two sources of beta-tocopherol in sunflower Helia 28 1–8

    Google Scholar 

  • R.E. Voorrips (2002) ArticleTitleMapChart: software for the graphical presentation of linkage maps and QTL J. Hered. 93 77–78 Occurrence Handle10.1093/jhered/93.1.77 Occurrence Handle1:CAS:528:DC%2BD38XktlOntrw%3D Occurrence Handle12011185

    Article  CAS  PubMed  Google Scholar 

  • J.K. Yu S. Tang M.B. Slabaugh A. Heesacker G. Cole M. Herring J. Soper F. Han W.C. Chu D.M. Webb L. Thompson K.J. Edwards S. Berry A.J. Leon C. Olungu N. Maes S.J. Knapp (2003) ArticleTitleTowards a saturated molecular genetic linkage map for cultivated sunflower Crop Sci. 43 367–387 Occurrence Handle1:CAS:528:DC%2BD3sXptVOksA%3D%3D

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Instituto de Agricultura Sostenible (CSIC), Apartado 4084, E-14080, Córdoba, Spain

    Elsa M. Vera-Ruiz, Leonardo Velasco, Jose M. Fernández-Martínez & Begoña Pérez-Vich

  2. Advanta Semillas, Ruta 226, Km 60.0., 7620 , Balcarce, Buenos Aires, Argentina

    Alberto J. Leon

Authors
  1. Elsa M. Vera-Ruiz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Leonardo Velasco
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alberto J. Leon
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jose M. Fernández-Martínez
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Begoña Pérez-Vich
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Begoña Pérez-Vich.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vera-Ruiz, E.M., Velasco, L., Leon, A.J. et al. Genetic Mapping of the Tph1 Gene Controlling Beta-tocopherol Accumulation in Sunflower Seeds. Mol Breeding 17, 291–296 (2006). https://doi.org/10.1007/s11032-005-5678-5

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11032-005-5678-5

Keywords

Search

Navigation