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Integration of Retrotransposons-Based Markers in a Linkage Map of Barley

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Abstract

A deeper understanding of random markers is important if they are to be employed for a range of objectives. The sequence specific amplified polymorphism (S-SAP) technique is a powerful genetic analysis tool which exploits the high copy number of retrotransposon long terminal repeats (LTRs) in the plant genome. The distribution and inheritance of S-SAP bands in the barley genome was studied using the Steptoe × Morex (S × M) double haploid (DH) population. Six S-SAP primer combinations generated 98 polymorphic bands, and map positions were assigned to all but one band. Eight putative co-dominant loci were detected, representing 16 of the mapped markers. Thus at least 81 of the mapped S-SAP loci were dominant. The markers were distributed along all of the seven chromosomes and a tendency to cluster was observed. The distribution of S-SAP markers over the barley genome concurred with the knowledge of the high copy number of retrotransposons in plants. This experiment has demonstrated the potential for the S-SAP technique to be applied in a range of analyses such as genetic fingerprinting, marker assisted breeding, biodiversity assessment and phylogenetic analyses.

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  1. Dipartimento di Scienze Agronomiche e Genetica Vegetale Agraria, Università degli Studi di Sassrai, Via E. De Nicola, 07100, Sassari, Italy

    Monica Rodriguez & Giovanna Attene

  2. Molecular Research Group, NIAB, Huntingdon Road, Cambridge, CB3 0LE, UK

    Donal O’Sullivan, Paolo Donini, Elena Chiapparino & Fiona Leigh

  3. Dipartimento di Scienze degli Alimenti, Università Politecnica delle Marche, Via Brecce Bianche, 60131, Ancona, Italy

    Roberto Papa

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  1. Monica Rodriguez
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  2. Donal O’Sullivan
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Correspondence to Monica Rodriguez.

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Rodriguez, M., O’Sullivan, D., Donini, P. et al. Integration of Retrotransposons-Based Markers in a Linkage Map of Barley. Mol Breeding 17, 173–184 (2006). https://doi.org/10.1007/s11032-005-4885-4

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