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Resistance Gene Analog Polymorphism (RGAP) Markers Co-Localize with Disease Resistance Genes and QTL in Common Bean

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Abstract

Resistance (R) genes containing nucleotide-binding site (NBS)-leucine rich repeats (LRR) are the most prevalent types of R gene in plants. The objective of this study was to develop PCR-based R-gene analog polymorphism (RGAP) markers for common bean (Phaseolus vulgaris L). Twenty degenerate primers were designed from the conserved kinase-1a (GVGKTT) and hydrophobic domains (GLPLAL) of known NBS-LRR type R-genes and from EST databases. Sixty-six of the 100 primer combinations tested yielded polymorphism. Thirty-two RGAP markers were mapped in the BAT 93/Jalo EEP558 core mapping population for common bean. The markers mapped to 10 of 11 linkage groups with a strong tendency for clustering. In addition, the RGAP markers co-located, on six linkage groups, with 15 resistance gene analogs (RGAs) that were previously mapped in other populations of common bean. The distance between the priming sites in NBS-LRR type R-genes is around 500 bp. Of the 32 RGAP markers, 19 had sizes larger and 13 less than 500 bp. RGAP markers mapped close to known R-genes on B11, and to QTLs for resistance on B1, B2, B6, B7, B8, B10, and B11. RGAP appears to provide a useful marker technique for tagging and mapping R-genes in segregating common bean populations, discovery of candidate genes underlying resistance QTL, and future cloning of R-genes in common bean.

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Abbreviations

HD:

Hydrophobic domain

K:

Kinase

LG:

Linkage group

QTL:

Quantitative trait locus/loci

RGAP:

Resistance gene analog polymorphism

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  1. Nedim Mutlu

    Present address: Bati Akdeniz Agricultural Research Institute, P.O. Box 35, 07100, Antalya, Turkey

Authors and Affiliations

  1. Department of Agronomy and Horticulture, University of Nebraska, Lincoln, NE, 68683, USA

    Nedim Mutlu & Dermot P. Coyne

  2. USDA-ARS, Vegetable and Forage Crop Research Unit, 24106 North Bunn Road, Prosser, WA, 99350, USA

    Phillip N. Miklas

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  1. Nedim Mutlu
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  2. Phillip N. Miklas
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Mutlu, N., Miklas, P.N. & Coyne, D.P. Resistance Gene Analog Polymorphism (RGAP) Markers Co-Localize with Disease Resistance Genes and QTL in Common Bean. Mol Breeding 17, 127–135 (2006). https://doi.org/10.1007/s11032-005-4474-6

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