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circPTK2 promotes proliferation, migration and invasion of trophoblast cells through the miR-619/WNT7B pathway in preeclampsia

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Abstract

It has been shown that the circular RNA (circRNA) circPTK2 modulates many types of diseases. However, the possible functions as well as the molecular mechanisms of circPTK2 in preeclampsia (PE) and their effects on trophoblast are unknown. Herein, we obtained the placental tissues from 20 pregnant women with PE who delivered in the Yueyang Maternal Child Medicine Health Hospital between 2019 and 2021 to serve as the PE group, and a normal group was composed of 20 healthy pregnant women with normal prenatal examinations. The circPTK2 level was significantly reduced in tissues from the PE group. The expression and localization of circPTK2 were verified using RT-qPCR. CircPTK2 silencing inhibited HTR-8/SVneo growth and migration in vitro. To investigate the underlying mechanism of circPTK2 in PE progression, dual-luciferase reporter assays were conducted. It was found that circPTK2 and WNT7B could bind directly to miR-619, and that circPTK2 affected WNT7B expression by sponging miR-619. To conclude, this study identified the functions and mechanisms of the circPTK2/miR-619/WNT7B axis in PE progression. In this way, circPTK2 has the potential to be used both in diagnostic and therapeutic settings for PE.

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The authors confirm that the data supporting the findings of this study are available within the article and its supplementary materials.

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Funding

This work was supported by the Research Foundation of Natural Science Foundation of Hunan province (No. 2021JJ40550); Natural Science Foundation of Hunan province (No. 2021JJ30693); National Natural Science Foundation of China(No. 82000421);Guiding project of Hengyang science and Technology Bureau(No. 2021181);Guiding project of Hengyang science and Technology Bureau(No. 201909);Natural Science Foundation of Hunan province (No. 2022JJ40016); Scientific Research Project of Hunan Provincial Health Commission (No.20200929); Clinical Innovation Leading Scientific Research Projects of Hunan Science and Technology Department (No.2020SK52601).

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Author notes

  1. Shiyuan Wu and Lingyan Liu equally contributed to this work.

Authors and Affiliations

  1. Scientific Research Institute, Yueyang Maternal-Child Medicine Health Hospital, Yueyang, China

    Shiyuan Wu, Ting Tao, Jingyan Xiao & Peng Wu

  2. Hengyang Maternal and Child Health Hospital, Hengyang, China

    Lingyan Liu, Jun Chen & Peng Wu

  3. Department of Gynaecology and Obstetrics, the Air Force Hospital of Southern Theater Command, Guangzhou, China

    Huizhi Yang, Xiaoshan Yu & Zhiqin Tan

Authors
  1. Shiyuan Wu
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  2. Lingyan Liu
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  3. Ting Tao
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  4. Jingyan Xiao
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  5. Huizhi Yang
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  6. Xiaoshan Yu
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  7. Jun Chen
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  8. Zhiqin Tan
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  9. Peng Wu
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Contributions

SW and LL designed and carried out the study, collected and analyzed the data, and wrote the manuscript. SW, TT and JX conducted the cell experiments. XY and JC performed glucose and lactate assay on. ZT and PW reviewed the manuscript. All authors read and approved the final manuscript.

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Correspondence to Zhiqin Tan or Peng Wu.

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Wu, S., Liu, L., Tao, T. et al. circPTK2 promotes proliferation, migration and invasion of trophoblast cells through the miR-619/WNT7B pathway in preeclampsia. Mol Cell Biochem 478, 2621–2627 (2023). https://doi.org/10.1007/s11010-023-04688-1

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  • DOI: https://doi.org/10.1007/s11010-023-04688-1

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