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The circ_FAM53B-miR-183-5p-CCDC6 axis modulates the malignant behaviors of papillary thyroid carcinoma cells

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Abstract

Papillary thyroid carcinoma (PTC) is a common thyroid malignancy. Circular RNAs (circRNAs) have been implicated in the development of PTC. Here, we explored the function and mechanism of circRNA family with sequence similarity 53, member B (circ_FAM53B) in PTC pathogenesis. Circ_FAM53B, microRNA (miR)-183-5p and coiled-coil domain containing 6 (CCDC6) levels were gauged by quantitative reverse transcription polymerase chain reaction (qRT-PCR) or Western blotting. The direct relationship between miR-183-5p and circ_FAM53B or CCDC6 was verified by dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. Our data showed that circ_FAM53B expression was reduced in PTC tissues and cells. Circ_FAM53B expression restrained proliferation, migration, and invasion and triggered apoptosis of PTC cells, as well as hindered HUVEC tube formation. Circ_FAM53B repressed miR-183-5p expression. MiR-183-5p re-expression reversed the effects of circ_FAM53B on cell behaviors. MiR-183-5p targeted and inhibited CCDC6, and circ_FAM53B upregulated CCDC6 through miR-183-5p competition. MiR-183-5p knockdown repressed cell proliferation, migration, invasion, and tube formation and facilitated apoptosis by upregulating CCDC6. Furthermore, circ_FAM53B reduced tumor growth in vivo. Collectively, our findings suggest that circ_FAM53B affects PTC cell biological behaviors via the miR-183-5p-CCDC6 axis.

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Data Availability

The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.

Abbreviations

PTC:

Papillary thyroid carcinoma

circRNAs:

Circular RNAs

CCDC6:

Coiled-coil domain containing 6

GEO:

GSE93522 Gene Expression Omnibus

UTR:

Untranslated region

PDCD4:

Programmed cell death 4

CREB1:

CAMP response element‑binding protein 1

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Acknowledgements

Not applicable.

Funding

This study was study was supported by Chongqing Natural Science Foundation Postdoctoral Science Foundation Project (Grant No. cstc2019jcyj-bshX0123) and Special postdoctoral projects in Chongqing (No: XmT2018066).

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Authors and Affiliations

  1. Department of Pathology, Chongqing university Fuling Hospital, No. 2, Gaosuntang Road, Chong Qing, 408099, China

    Chong Zhang, Dingrong Liu, Fuyun Tong, Huijie Wei, Dan Zhou, Jing Fang, Xiaolu Dai & Haibo Tian

  2. Department of Network Information, Chongqing university Fuling Hospital, Chong Qing, 408099, China

    Huxia Gu

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  1. Chong Zhang
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All authors made substantial contribution to conception and design, acquisition of the data, or analysis and interpretation of the data; take part in drafting the article or revising it critically for important intellectual content; gave final approval of the revision to be published; and agree to be accountable for all aspect of the work.

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Zhang, C., Gu, H., Liu, D. et al. The circ_FAM53B-miR-183-5p-CCDC6 axis modulates the malignant behaviors of papillary thyroid carcinoma cells. Mol Cell Biochem 477, 2627–2641 (2022). https://doi.org/10.1007/s11010-022-04465-6

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