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Effects of induced pluripotent stem cells-derived conditioned medium on the proliferation and anti-apoptosis of human adipose-derived stem cells

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Abstract

Human adipose-derived stem cells (hASCs) become an appealing source for regenerative medicine. However, with the multi-passage or cryopreservation for large-scale growth procedures in terms of preclinical and clinical purposes, hASCs often reveal defective cell viability, which is a major obstacle for cell therapy. In our study, the effects of induced pluripotent stem cells-derived conditioned medium (iPS-CM) on the proliferation and anti-apoptosis in hASCs were investigated. hASCs at passage 1 were identified by the analysis of typical surface antigens with flow cytometry assay and adipogenic and osteogenic differentiation. The effect of iPS-CM on the proliferation in hASCs was analyzed by cell cycle assay and Ki67/P27 quantitative polymerase chain reaction analysis. The effect of iPS-CM on the anti-apoptosis of hASCs irradiated by 468 J/m2 of ultraviolet C was investigated by annexin v/propidium iodide analysis, mitochondrial membrane potential assay, intracellular reactive oxygen species assay, Western blotting and caspase activity assays. The effect of iPS-CM on the surface antigen expressions of hASCs was analyzed using flow cytometry assay. The levels of Activin A and bFGF in culture supernatant of hASCs with different treatments were also detected by enzyme-linked immunosorbent assay. iPS-CM promoted proliferation and inhibited apoptosis of hASCs. This discovery demonstrates that iPS-CM might be used as one of the available means to overcome the propagation obstacle for hASCs and make for large-scale growth procedures in terms of preclinical and clinical purposes.

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Acknowledgments

This study was supported by National Natural Science Foundation of China (No. 81371689), collaborated grant for HK-Macao-TW of Ministry of Science and Technology (2012DFH30060) and Special Funds for Major Science and Technology Projects of Guangdong Province (2015B010125007).

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    Authors and Affiliations

    1. Department of Ophthalmology, First Affiliated Hospital of Jinan University, Guangzhou, 510632, Guangdong, China

      Rui-Ling Lian & Jian-Su Chen

    2. Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou, 510632, Guangdong, China

      Xiao-Ling Guo, Jian-Su Chen & Yong-Long Guo

    3. Institute of Ophthalmology, Medical College of Jinan University, #601 West Huangpu Avenue, Guangzhou, 510632, Guangdong, China

      Jian-Su Chen

    4. Department of Biomedical Engineering, Jinan University, Guangzhou, 510632, Guangdong, China

      Jia-Fu Zheng

    5. Department of Plastic Surgery, First Affiliated Hospital of Jinan University, Guangzhou, 510632, Guangdong, China

      Yuan-Wen Chen

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    Rui-Ling Lian and Xiao-Ling Guo have contributed equally to this work.

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    Lian, RL., Guo, XL., Chen, JS. et al. Effects of induced pluripotent stem cells-derived conditioned medium on the proliferation and anti-apoptosis of human adipose-derived stem cells. Mol Cell Biochem 413, 69–85 (2016). https://doi.org/10.1007/s11010-015-2640-7

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