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Identification of microRNAs involved in dexamethasone-induced muscle atrophy

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Abstract

MicroRNAs (miRNAs), a novel class of post-transcriptional gene regulators, have been demonstrated to be involved in several cellular processes regulating the expression of protein-coding genes. To investigate the mechanisms of miRNA-mediated regulation during the process of muscle atrophy, we performed miRNA microarray hybridization between normal differentiated C2C12 cells and dexamethasone (DEX)-treated C2C12 cells. We observed that 11 miRNAs were significantly up-regulated and six miRNAs were down-regulated in the differentiated C2C12 cells after being treated with DEX. Stem–loop real-time RT-PCR confirmed the differential expression of six selected miRNAs (miR-1, miR-147, miR-322, miR-351, and miR-503*, miR-708). miRNA potential target prediction was accomplished using TargetScan, and many target genes related to muscle growth and atrophy have been reported in previous studies. The results of the current study suggested the potential roles of these differentially expressed miRNAs in skeletal muscle atrophy.

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Acknowledgments

This study was supported by the National Outstanding Youth Foundation of NSFC (31025026), the NSFC youth foundation (30900792), the creative team project of the Ministry of Education of China (IRT-0831), and the National Key Basic Research Program of China (2012CB124702).

Conflict of interests

The authors have declared that no competing interest exists.

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  1. Key Laboratory of Agricultural Animal Genetics, Breeding & Reproduction of Ministry of Education, Key Laboratory of Swine Genetics & Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    He Shen, Teng Liu, Liangliang Fu, Shuhong Zhao, Bin Fan, Jianhua Cao & Xinyun Li

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  1. He Shen
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Correspondence to Xinyun Li.

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Shen, H., Liu, T., Fu, L. et al. Identification of microRNAs involved in dexamethasone-induced muscle atrophy. Mol Cell Biochem 381, 105–113 (2013). https://doi.org/10.1007/s11010-013-1692-9

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