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Adipogenic and osteogenic differentiation of Lin−CD271+Sca-1+ adipose-derived stem cells

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Abstract

Adipose-derived stem cells (ASCs) have been defined as cells that undergo sustained in vitro growth and have multilineage differentiation potential. However, the identity and purification of ASCs has proved elusive due to the lack of specific markers and poor understanding of their physiological roles. Here, we prospectively isolated and identified a restricted homogeneous subpopulation of ASCs (Lin−CD271+Sca-1+) from mouse adipose tissues on the basis of cell-surface markers. Individual ASCs generated colony-forming unit-fibroblast at a high frequency and could differentiate into adipocytes, osteoblasts, and chondrocytes in vitro. Expansion of ASCs in a large quantity was feasible in medium supplemented with fibroblast growth factor-2 and leukemia inhibitory factor, without loss of adipogenic and osteogenic differentiation capacity. Moreover, we found that the transplanted ASCs can differentiate into adipocytes in adipogenic microenvironment in vivo and osteoblasts in osteogenic microenvironment in vivo. Thus we proved that Lin, CD271, and Sca-1 could be used as the specific markers to purify ASCs from adipose tissue. The method we established to identify ASCs as defined in vivo entities will help develop ASCs transplantation as a new therapeutic strategy for bone regeneration and adipose tissue regeneration in clinic.

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Acknowledgments

We thank Prof. Xianming Mo of the Laboratory of Stem Cell Biology, State Key Laboratory of Biotherapy for help. This work was supported by grants from the National Natural Science Foundation of China (30973348), the Chinese Ministry of Science and Technology under Contract Preliminary Project (2006CB708505), and the Chinese National 973 projects (2010CB944800) to Weidong Tian. It was also supported by grants from the National Basic Research Program of China (2007CB947802) to Wentong Meng.

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Authors and Affiliations

  1. State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, 610041, China

    Jingang Xiao, Xiaojuan Yang, Wei Jing, Weihua Guo, Qince Sun, Yunfeng Lin, Lei Liu & Weidong Tian

  2. Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Luzhou Medical College, Luzhou, 646000, China

    Jingang Xiao

  3. Laboratory of Stem Cell Biology, State Key Laboratory of Biotherapy, Sichuan University, Chengdu, 610041, China

    Wentong Meng

  4. Department of Oral and Maxillofacial Surgery, Western China Stomatological Hospital, Sichuan University, Chengdu, 610041, China

    Weidong Tian

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  1. Jingang Xiao
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  2. Xiaojuan Yang
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  3. Wei Jing
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  4. Weihua Guo
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  5. Qince Sun
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  6. Yunfeng Lin
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  8. Wentong Meng
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  9. Weidong Tian
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Correspondence to Wentong Meng or Weidong Tian.

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Xiao, J., Yang, X., Jing, W. et al. Adipogenic and osteogenic differentiation of Lin−CD271+Sca-1+ adipose-derived stem cells. Mol Cell Biochem 377, 107–119 (2013). https://doi.org/10.1007/s11010-013-1575-0

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  • DOI: https://doi.org/10.1007/s11010-013-1575-0

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