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Vitamin E slows the progression of hypercholesterolemia-induced oxidative stress in heart, liver and kidney

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Abstract

Vitamin E suppresses the hypercholesterolemia-induced oxidative stress in the heart. The objectives were to investigate if: (a) hypercholesterolemia-induced oxidative stress is similar in heart, liver, and kidney, and is dependent upon duration of hypercholesterolemia; and (b) vitamin E slows the progression of oxidative stress in these organs. The rabbits were assigned to 4 groups: I, regular diet (2 months); II, 0.25 % cholesterol diet (2 months); III, 0.25 % cholesterol diet (4 months); and IV, 0.25 % cholesterol diet (2 months) followed by 0.25 % cholesterol diet plus vitamin E (2 months). Blood samples were collected before and at the end of protocol for the measurement of total cholesterol (TC). Hearts, livers, and kidneys were removed at the end of the protocol under anesthesia for the measurement of oxidative parameters, malondialdehyde (MDA), and chemiluminescence (CL). The basal MDA levels in the heart, liver, and kidney of rabbits in Group I were similar, but increased to 14.65-, 3.18-, and 10.35-fold, respectively, with hypercholesterolemia. The increases in MDA levels were dependent upon the duration of hypercholesterolemia. Vitamin E did not alter the TC levels, but reduced the MDA levels in all organs. Hypercholesterolemia and vitamin E had variable effects on CL activity. In conclusion, (i) hypercholesterolemia induces oxidative stress in heart, liver, and kidney, the heart being the most and the liver the least susceptible to oxidative stress; (ii) oxidative stress is positively associated with duration of hypercholesterolemia; and (iii) vitamin E slows the progression of oxidative stress in these organs.

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References

  1. Prasad K, Kalra J (1993) Oxygen free radicals and hypercholesterolemic atherosclerosis: effect of vitamin E. Am Heart J 125:958–973

    Article  PubMed  CAS  Google Scholar 

  2. Prasad K, Kalra J, Lee P (1994) Oxygen free radicals as a mechanism of hypercholesterolemic atherosclerosis: effects of probucol. Int J Angiol 3:100–112

    Article  Google Scholar 

  3. Prasad K (1999) Reduction of serum cholesterol and hypercholesterolemic atherosclerosis in rabbits by secoisolariciresinol diglucoside isolated from flaxseed. Circulation 99:1355–1362

    Article  PubMed  CAS  Google Scholar 

  4. Prasad K, Lee P (2007) Suppression of hypercholesterolemic atherosclerosis by pentoxifylline and its mechanism. Atherosclerosis 192:313–322

    Article  PubMed  CAS  Google Scholar 

  5. Prasad K (2005) Hypocholesterolemic and antiatherosclerotic effect of flax lignan complex isolated from flaxseed. Atherosclerosis 179:269–275

    Article  PubMed  CAS  Google Scholar 

  6. Ohara Y, Peterson TE, Harrison DG (1993) Hyperchlesterolemia increases endothelial superoxide anion production. J Clin Invest 91:2546–2551

    Article  PubMed  CAS  Google Scholar 

  7. Prasad K (1997) Dietary flaxseed in the prevention of hypercholesterolemic atherosclerosis. Atherosclerosis 132:69–76

    Article  PubMed  CAS  Google Scholar 

  8. Prasad K, Lee P, Mantha SV et al (1992) Detection of ischemia–reperfusion cardiac injury by cardiac muscle chemiluminescence. Mol Cell Biochem 115:49–58

    Article  PubMed  CAS  Google Scholar 

  9. Freeman BA, Crapo JD (1982) Biology of disease: free radical and tissue injury. Lab Invest 47:412–426

    PubMed  CAS  Google Scholar 

  10. Prasad K, Gupta JB, Kalra J et al (1996) Oxidative stress as a mechanism of cardiac failure in chronic volume overload in canine model. J Mol Cell Cardiol 28:375–385

    Article  PubMed  CAS  Google Scholar 

  11. Ferrari R, Angoletti L, Comini L et al (1998) Oxidative stress during myocardial ischemia and heart failure. Eur Heart J 19(Suppl B):B2–B11

    PubMed  CAS  Google Scholar 

  12. Jarrar D, Wang P, Cioffi WG et al (2000) Critical role of oxygen radicals in the initiation of hepatic depression after trauma hemorrhage. J Trauma 49:879–885

    Article  PubMed  CAS  Google Scholar 

  13. Poli G (1993) Liver damage due to free radicals. Br Med Bull 49:604–620

    PubMed  CAS  Google Scholar 

  14. Galle JC (2001) Oxidative stress in chronic renal failure. Nephrol Dial Transplant 16:2135–2137

    Article  PubMed  CAS  Google Scholar 

  15. Baker GL, Correy RJ, Autor AP (1995) Oxygen free radical-induced damage in kidneys subjected to warm ischemia and reperfusion. Protective effects of superoxide dismutase. Ann Surg 202:628–641

    Article  Google Scholar 

  16. Burton GW, Ingold KU (1989) Vitamin E as an in vitro and in vivo antioxidant. Ann NY Acad Sci 570:7–22

    Article  PubMed  CAS  Google Scholar 

  17. Prasad K, Mantha SV, Kalra J, Lee P (1997) Hypercholesterolemia-induced oxidative stress in heart and its prevention by vitamin E. Int J Angiol 6:13–17

    Article  Google Scholar 

  18. Prasad K, Mantha SV, Kalra J, Kapoor R et al (1997) Purpuragallin in the prevention of hypercholesterolemic atherosclerosis. Int J Angiol 6:157–166

    Article  Google Scholar 

  19. Kapoor R, Prasad K (1994) Role of oxyradicals in cardiovascular depression and cellular injury in hemorrhagic shock and reinfusion: effect of SOD and catalase. Circ Shock 43:79–94

    PubMed  CAS  Google Scholar 

  20. Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal Biochem 95:351–358

    Article  PubMed  CAS  Google Scholar 

  21. Gornall AG, Bardawill CJ, David MM (1949) Determination of serum proteins by means of biuret reaction. J Biol Chem 177:751–756

    PubMed  CAS  Google Scholar 

  22. Prasad K (2009) Flax lignin complex slows down the progression of atherosclerosis in hyperlipidemic rabbits. J Cardiovasc Pharmacol Ther 14:38–48

    Article  PubMed  CAS  Google Scholar 

  23. Averill MM, Bennett BJ, Ratazzi M et al (2009) Neither antioxidants nor genistein inhibit the progression of established atherosclerotic lesions in older apoE deficient mice. Atherosclerosis 203:82–88

    Article  PubMed  CAS  Google Scholar 

  24. Csont T, Bereczki E, Bencsik P et al (2007) Hypercholesterolemia increases myocardial oxidative and nitrosative stress thereby leading to cardiac dysfunction in apoB-100 transgenic mice. Cardiovasc Res 76:100–109

    Article  PubMed  CAS  Google Scholar 

  25. Kucukegergin C, Aydn F, Ozdermirler-Erata G et al (2010) Effect of artichoke leaf extract on hepatic and cardiac oxidative stress in rats fed on a high cholesterol diet. Biol Trace Elem Res 135:264–274

    Article  Google Scholar 

  26. Rodriguez-Porcel M, Lerman A, Best PJM et al (2001) Hypercholesterolemia impairs myocardial perfusion and permeability: role of oxidative stress and endogenous scavenging activity. J Am Coll Cardiol 37:608–615

    Article  PubMed  CAS  Google Scholar 

  27. Mateos R, Lecumberri E, Ramos S et al (2005) Determination of malondialdehyde (MDA) by high performance liquid chromatography in serum and liver as a biomarker for oxidative stress. Application to a rat model for hypercholesterolemia and evaluation of the effect of diets rich in phenolic antioxidants from fruits. J Chromatogr Biomed Life Sci 827:76–82

    Article  CAS  Google Scholar 

  28. Mahfouz MM, Kummerow FA (2007) Cholesterol rich-diets have different effects on lipid peroxidation, cholesterol oxide, and antioxidant enzymes in rats and rabbits. J Nutr Biochem 15:293–302

    Google Scholar 

  29. Prasanna GS, Purnima A (2011) Protective effects of leaf extract of Trichilia connaroides on hypercholesterolemia induced oxidative stress. Int J Pharmacol 7:106–112

    Article  Google Scholar 

  30. Godin DV, Dahlman DM (1993) Effects of hypercholesterolemia on tissue antioxidant status in two species differing in susceptibility to atherosclerosis. Res Commun Chem Pathol Pharmacol 79:151–156

    PubMed  CAS  Google Scholar 

  31. Yang D, Lin S, Yang D et al (2012) Effects of short- and long-term hypercholesterolemia on contrast-induced acute kidney injury. Am J Nephrol 35:80–89

    Article  PubMed  CAS  Google Scholar 

  32. Lee HS, Jeong JY, Kim BC et al (1997) Dietary antioxidants inhibit lipoprotein oxidation and renal injury in experimental focal segmental glomerulosclerosis. Kidney Int 51:1151–1159

    Article  PubMed  CAS  Google Scholar 

  33. Minami M, Ishiyama A, Takagi M et al (2005) Effects of allopurinol, a xanthine oxidase inhibitor, on renal injury in hypercholesterolemia-induced hypertensive rats. Blood Press 14:120–125

    Article  PubMed  CAS  Google Scholar 

  34. Scheuer H, Gwinner W, Honbach J et al (2000) Oxidant stress in hypercholesterolemia-induced renal damage. Am J Physiol Renal Physiol 278:F63–F74

    PubMed  CAS  Google Scholar 

  35. Vega-Warner V, Ransom RF, Vincent AM et al (2004) Induction of antioxidant enzymes in murine podocytes precedes injury by puromycin aminonucleoside. Kidney Int 66:1881–1889

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This study has been supported by a grant from the Heart and Stroke Foundation of Saskatchewan and College of medicine Research Fund of the University of Saskatchewan, Saskatoon, Canada. The technical assistance of Mrs. Barbara Raney is highly appreciated.

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Authors and Affiliations

  1. Department of Physiology, College of medicine, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK, S7N 5E5, Canada

    Kailash Prasad

  2. Department of Surgery, University of Saskatchewan and Royal University Hospital, Saskatoon, SK, Canada

    Erick D. McNair

  3. Pathology and Laboratory Medicine, University of Saskatchewan and Royal University Hospital, Saskatoon, SK, Canada

    A. Mabood Qureshi

  4. Department of Medicine, University of Saskatchewan and Royal University Hospital, Saskatoon, SK, Canada

    Gudrun Casper-Bell

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  1. Kailash Prasad
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  2. Erick D. McNair
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  3. A. Mabood Qureshi
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  4. Gudrun Casper-Bell
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Correspondence to Kailash Prasad.

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Prasad, K., McNair, E.D., Qureshi, A.M. et al. Vitamin E slows the progression of hypercholesterolemia-induced oxidative stress in heart, liver and kidney. Mol Cell Biochem 368, 181–187 (2012). https://doi.org/10.1007/s11010-012-1358-z

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  • DOI: https://doi.org/10.1007/s11010-012-1358-z

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