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Neuroprotection against cobalt chloride-induced cell apoptosis of primary cultured cortical neurons by salidroside

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Abstract

Salidroside, a phenol glycoside of plant origin, has been documented to possess a broad spectrum of pharmacological properties, including protective effects against neuronal death induced by different insults. To provide further insights into the neuroprotective functions peculiar to salidroside, this study used primary cultured cortical neurons of rats as a cell model to examine whether salidroside was able to prevent against cell damage after exposure to cobalt chloride (CoCl2), a hypoxia-inducing agent. The data from 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test, Hoechst33342 staining, terminal deoxynucleotidyl transferase dUTP-mediated nicked end labeling assay, and Bax/Bcl-2 ratio analysis indicated that salidroside pretreatment attenuated hypoxia-induced apoptotic cell death of primary cultured cortical neurons in a dose-dependent manner. Moreover, preliminary exploration of the possible mechanisms suggested that the protective effects of salidroside, shown in our experimental setting, might probably be mediated by enhancing the expression of hypoxia-inducible factor-1α, alleviating the increase of intracellular reactive oxygen species levels, and inhibiting over-expression of nuclear factor-kappa B protein.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (Grant No. 30870881), a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, and the key project of Jiangsu Education Department (Grant No. 08KJA310002).

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu, People’s Republic of China

    Shuqiang Zhang, Xia Chen, Yumin Yang, Xinyang Zhou, Jie Liu & Fei Ding

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  1. Shuqiang Zhang
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Correspondence to Fei Ding.

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Zhang, S., Chen, X., Yang, Y. et al. Neuroprotection against cobalt chloride-induced cell apoptosis of primary cultured cortical neurons by salidroside. Mol Cell Biochem 354, 161–170 (2011). https://doi.org/10.1007/s11010-011-0815-4

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  • DOI: https://doi.org/10.1007/s11010-011-0815-4

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