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Protective effect of salidroside against H2O2-induced cell apoptosis in primary culture of rat hippocampal neurons

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Abstract

Salidroside, a phenylpropanoid glycoside separated from a medicinal plant Rhodiola rosea, has been documented to have protective effects on neuronal cells in vitro. This study investigated whether salidroside was able to extend its unique neuroprotection to primary cultured rat hippocampal neurons against hydrogen peroxide (H2O2)-induced cell damage. Cell viability tests and cell apoptosis assays confirmed that salidroside pretreatment attenuated H2O2-stimulated apoptotic cell death in primary culture of hippocampal neurons in a concentration-dependent manner. The measurements of caspase-3 activity, nitric oxide (NO) production, and NO synthase (NOS) activity suggest that the protection of salidroside, shown in this study, might be mediated by inhibiting caspase-3 activity, and antagonizing NO production and NOS activity during H2O2 stimulation. Perhaps, this study might contribute to the development of salidroside as a broad-spectrum agent for preventing and/or treating neuronal damage in neurodegenerative disorders.

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Acknowledgments

This study was supported by Hi-Tech Research and Development Program of China (973 Program, Grant No. 2003CB515306). We thank Professor Jie Liu for assistance in manuscript preparation.

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Authors and Affiliations

  1. Medical School of Soochow University, JS 215123, Suzhou, People’s Republic of China

    Xia Chen

  2. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, JS 226001, Nantong, People’s Republic of China

    Xia Chen, Qi Zhang, Qiong Cheng & Fei Ding

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  1. Xia Chen
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  2. Qi Zhang
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  4. Fei Ding
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Correspondence to Fei Ding.

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Chen, X., Zhang, Q., Cheng, Q. et al. Protective effect of salidroside against H2O2-induced cell apoptosis in primary culture of rat hippocampal neurons. Mol Cell Biochem 332, 85–93 (2009). https://doi.org/10.1007/s11010-009-0177-3

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  • DOI: https://doi.org/10.1007/s11010-009-0177-3

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