Abstract
Matrix metalloproteinases (MMPs) are key elements in extracellular matrix (ECM) degradation and scar remodeling during the wound-healing process. Our previous data revealed that keratinocyte-releasable factors significantly increased the expression of fibroblast MMPs in monolayer-cultured fibroblasts. In this study, we analyzed the differences in the MMP expressions of fibroblasts in a three-dimensional fibroblast-populated collagen gel (3D FPCG) from that in a two-dimensional monolayer-cultured fibroblasts when both co-cultured with keratinocytes. Differential mRNA and protein expression of fibroblasts were examined by microarray, RT-PCR, and western blot. Our results showed that fibroblasts co-cultured with keratinocytes in a 3D FPCG expressed significantly higher MMP1 and MMP3 at the gene and protein levels. Due to the physiological advantages of a 3D FPCG model to a 2D system, we concluded that the 3D FPCG model may provide a better means of understanding the fibroblast–keratinocyte cross-talk during the wound-healing process.
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Acknowledgements
This study has been supported by a grant funded by the Canadian Institute of Health Research. We thank Dr. Ruhangiz Taghi Kilani, Dr. Abdi Ghaffari, and Dr. Reza B. Jalili for excellent technique support.
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Li, M., Moeen Rezakhanlou, A., Chavez-Munoz, C. et al. Keratinocyte-releasable factors increased the expression of MMP1 and MMP3 in co-cultured fibroblasts under both 2D and 3D culture conditions. Mol Cell Biochem 332, 1–8 (2009). https://doi.org/10.1007/s11010-009-0167-5
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DOI: https://doi.org/10.1007/s11010-009-0167-5