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Differential roles of MAPKs and MSK1 signalling pathways in the regulation of c-Jun during phenylephrine-induced cardiac myocyte hypertrophy

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Abstract

Gq-protein-coupled receptor (GqPCR) signalling is associated with the induction of cardiac myocyte hypertrophy, which is characterized by an increase in expression of immediate early genes via activation of pre-existing transcription factors. Here, we explore the role of MSK1 and MAPK signalling pathways in the regulation of the immediate early gene c-jun. The results provide further support for the role of MSK1 in cardiac myocyte hypertrophy and indicate that PE activates distinct signalling mechanisms which culminate with a complex activation of c-jun. ERK1/2 and JNKs are the principal kinases responsible for phosphorylation of c-Jun, whereas c-jun mRNA and protein up-regulation by PE is mediated by multiple signalling pathways that include MSK1, ERK1/2, p38-MAPK and JNKs. These signalling mechanisms seem to be critical to the phenotypic changes of cardiac myocytes in response to hypertrophic stimulation.

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Acknowledgements

This work was partially supported by a grant from Empirikion Foundation.

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Authors and Affiliations

  1. Laboratory of Animal Physiology, School of Biology, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece

    Thomais Markou, Danuta Cieslak & Antigone Lazou

  2. Department of Animal and Human Physiology, School of Biology, University of Athens, Athens, 157 84, Greece

    Catherine Gaitanaki

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  1. Thomais Markou
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  2. Danuta Cieslak
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  4. Antigone Lazou
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Correspondence to Antigone Lazou.

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Markou, T., Cieslak, D., Gaitanaki, C. et al. Differential roles of MAPKs and MSK1 signalling pathways in the regulation of c-Jun during phenylephrine-induced cardiac myocyte hypertrophy. Mol Cell Biochem 322, 103–112 (2009). https://doi.org/10.1007/s11010-008-9945-8

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