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NF-κB controls growth of glioblastomas/astrocytomas

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Abstract

NF-κB is a family of transcription factors that have been shown to be elevated in a variety of tumor types and in some cases central to their survival and growth. Here we present evidence that U-87 MG and U-118 MG growth is regulated by NF-κB and controlled by PDGF. NF-κB activity was suppressed by a dominant negative mutant of the human PDGF type β receptor and PDGF-B chain neutralizing antibodies. Creation of cell lines that had inducible expression of shRNAs directed against either c-Rel or RelA inhibited growth almost 90% indicating that NF-κB plays a central role in glioblastoma growth.

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Acknowledgements

The authors would like to acknowledge Dr. John Geiser for critical reading of the manuscript and Dr. Todd Barkman for sequencing. This work was funded by grants from the National Institutes of Health and the Elsa U. Pardee Foundation.

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Authors and Affiliations

  1. Department of Biological Sciences, Western Michigan University, Kalamazoo, MI, 49008, USA

    Denise Smith, Stephanie Barbera & Bruce E. Bejcek

  2. Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, 02115, USA

    Takeshi Shimamura

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  1. Denise Smith
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  2. Takeshi Shimamura
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  4. Bruce E. Bejcek
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Correspondence to Bruce E. Bejcek.

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Smith, D., Shimamura, T., Barbera, S. et al. NF-κB controls growth of glioblastomas/astrocytomas. Mol Cell Biochem 307, 141–147 (2008). https://doi.org/10.1007/s11010-007-9593-4

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  • DOI: https://doi.org/10.1007/s11010-007-9593-4

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