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NTPDase and 5′-nucleotidase activities in platelets of human pregnants with a normal or high risk for thrombosis

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Abstract

The nucleotide degrading enzymes, ectonucleotidases, present on the platelet surface of human pregnant with a normal (without complications) or high risk for thrombosis (hypertension and gestational diabetes) were studied. NTPDase (E.C. 3.6.1.5, CD39) and 5′-nucleotidase (E.C. 3.1.3.5, CD73) activities of four patient groups, non-pregnant (NP, n = 18), pregnant without complications (P, n = 25), pregnant with hypertension (HP, n = 15) and pregnant with gestational diabetes mellitus (GDP, n = 10), were analyzed. Increased NTPDase activities were observed in the groups P (37.0%, S.D. = 2.03 and 34.0%, S.D. = 3.19), HP (40.0%, S.D. = 3.32 and 56.0%, S.D. = 3.25) and GDP (23.0%, S.D. = 2.30 and 42.0%, S.D. = 2.26) in comparison to the control group NP (p < 0.01, S.D. = 1.92 and S.D. = 2.48) when ATP and ADP were used as substrate, respectively. AMP was used as substrate to determine the 5′-nucleotidase activities, which showed to be elevated in the groups P (45.0%, S.D. = 1.73), HP (54.0%, S.D. = 2.64) and GDP (68.0%, S.D. = 1.69) when compared to the control group NP (p < 0.01, S.D. = 1.26). However, no statistically significant differences were observed between the groups P, HP and GDP. As a consequence, the enhanced ATP, ADP and AMP hydrolysis was ascribed to the pregnancy itself, independent of a normal or high risk for thrombosis. The enhanced NTPDase and 5′-nucleotidase activities in platelets suggest that these enzymes are involved in the thromboregulation process in the pregnancy.

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Authors and Affiliations

  1. Departamento de Análises Clínicas e Toxicológicas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Av. Roraima, Santa Maria, RS, 97105-900, Brazil

    Claudio A. M. Leal, Karine Bauchspiess, Clarissa M. L. Schrekker & José E. P. da Silva

  2. Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av. Roraima, Santa Maria, RS, 97105-900, Brazil

    Maria R. C. Schetinger, Daniela B. R. Leal, Paula A. Maldonado & Vera M. Morsch

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  1. Claudio A. M. Leal
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  2. Maria R. C. Schetinger
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  3. Daniela B. R. Leal
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  4. Karine Bauchspiess
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  5. Clarissa M. L. Schrekker
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  6. Paula A. Maldonado
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  7. Vera M. Morsch
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  8. José E. P. da Silva
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Correspondence to Maria R. C. Schetinger.

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Leal, C.A.M., Schetinger, M.R.C., Leal, D.B.R. et al. NTPDase and 5′-nucleotidase activities in platelets of human pregnants with a normal or high risk for thrombosis. Mol Cell Biochem 304, 325–330 (2007). https://doi.org/10.1007/s11010-007-9515-5

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  • DOI: https://doi.org/10.1007/s11010-007-9515-5

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