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Effects of uridine and nucleotides on hemostasis parameters

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Abstract

Several purinergic receptors have been identified on platelets which are involved in hemostatic and thrombotic processes. The aim of the present study was to investigate the effects of uridine and its nucleotides on platelet aggregation and hemostasis in platelet-rich plasma (PRP) and whole blood. The effects of uridine, UMP, UDP, and UTP at different final concentrations (1 to 1000 µM) on platelet aggregation were studied using an aggregometer. In PRP samples, platelet aggregation was induced by ADP, collagen and epinephrine 3 min after addition of uridine, UMP, UDP, UTP and saline (as a control). All thromboelastogram experiments were performed at 1000 µM final concentrations of uridine and its nucleotides in whole blood. UDP and UTP were also tested in thromboelastogram with PRP. Our results showed that UDP, and especially UTP, inhibited ADP- and collagen-induced aggregation in a concentration-dependent manner. In whole blood thromboelastogram experiments, UDP stimulated clot formation while UTP suppressed clot formation. When thromboelastogram experiments were repeated with PRP, UTP’s inhibitory effect on platelets was confirmed, while UDP’s stimulated clot forming effect disappeared. Collectively, our data showed that UTP inhibited platelet aggregation in a concentration-dependent manner and suppressed clot formation. On the other hand, UDP exhibited distinct effects on whole blood or PRP in thromboelastogram. These data suggest that the difference on effects of UTP and UDP might have arisen from the different receptors that they stimulate and warrant further investigation with regard to their in vivo actions on platelet aggregation and hemostasis.

Highlights

UDP and UTP exhibit different effects on platelet aggregation and hemostasis.

UTP inhibits platelet aggregation and hemostasis both in whole blood and PRP samples.

UDP has distinct effects on hemostasis in whole blood or PRP in thromboelastogram.

The findings suggest the involvement of different receptors stimulated by UDP or UTP in the observed effects.

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Funding

This work was supported by the Bursa Uludağ University Scientific Research Projects Unit under Grant BUAP(T) − 2015/2.

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Authors and Affiliations

  1. Vocational School of Health Services, KTO Karatay University, Konya, Turkey

    Merve Arı

  2. School of Medicine, Department of Biophysics, Bursa Uludağ University, Bursa, 16059, Turkey

    Engin Sağdilek

  3. School of Medicine, Department of Biophysics, Acıbadem University, İstanbul, Turkey

    Evren Kılınç

  4. School of Medicine, Department of Pharmacology, Bursa Uludağ University, Bursa, Turkey

    Mehmet Cansev

  5. School of Medicine, Department of Physiology, Bursa Uludağ University, Bursa, Turkey

    Kasım Özlük

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  1. Merve Arı
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Correspondence to Engin Sağdilek.

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Arı, M., Sağdilek, E., Kılınç, E. et al. Effects of uridine and nucleotides on hemostasis parameters. J Thromb Thrombolysis 55, 626–633 (2023). https://doi.org/10.1007/s11239-023-02793-y

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