Abstract
Phosphorylation status of RNA polymerase (RNAP) II’s largest subunit C-terminal domain (CTD) plays an important role during transcription cycles. The reversible phosphorylation mainly occurs at serine 2 and serine 5 of CTD heptapeptide repeats and regulates RNAP II’s activity during transcription initiation, elongation and RNA processing. Here we expressed and characterized HSPC129, a putative human protein bearing a CTD phosphatase domain (CPD). PCR analysis showed that it was ubiquitously expressed. HSPC129ΔTM, the truncate HSPC129 with first 156 N terminal amino acids deleted, exhibited Mg2+ dependent phosphatase activity at pH 5.0. Its specific CTD phosphatase activity was verified in vitro. Our research suggests that HSPC129 may regulate the dynamic phosphorylation of RNAP II CTD.
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Acknowledgements
This work is supported by Grants from the 863 Projects of Ministry of Science and Technology of P.R. China (No. 2003AA221020) and Key project of National Natural Science Foundation of China (No. 10490190 & 10490193).
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Qian, H., Ji, C., Zhao, S. et al. Expression and characterization of HSPC129, a RNA polymerase II C-terminal domain phosphatase. Mol Cell Biochem 303, 183–188 (2007). https://doi.org/10.1007/s11010-007-9472-z
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DOI: https://doi.org/10.1007/s11010-007-9472-z