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The effect of Na+-K+ ATPase inhibition by ouabain on histamine release from human cutaneous mast cells

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Abstract

Background: There are controversial reports on the effect of sodium-potassium adenosine triphosphatase (Na+-K+ ATPase) inhibition on mast cell mediator release. Some of them have indicated that ouabain (strophanthin G), a specific Na+-K+ ATPase inhibitor, inhibited the release, whereas the others have shown that ouabain had no effect or even had a stimulatory effect on the mediator secretion. Most of these studies have utilized animal-derived mast cells. The aim of this study was to determine the effect of Na+-K+ ATPase inhibition on human skin mast cells.

Methods: Unpurified and purified mast cells were obtained from newborn foreskins and stimulated by calcium ionophore A23187 (1 μM) for 30 min following a 1 hr incubation with various concentrations (10−4 to 10−8 M) of ouabain. Histamine release was assayed by enzyme-linked immunosorbent assay (ELISA).

Results: The results indicated that ouabain had no significant effect on the non-immunologic histamine release from human skin mast cells, in vitro.

Conclusions: Na+-K+ ATPase inhibition by ouabain had no significant effect on the non-immunologic histamine release from human cutaneous mast cells and suggested differences between human and animal mast cells.

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Authors and Affiliations

  1. Departments of Dermatology, Inonu University School of Medicine, 44069, Malatya, Turkey

    Mustafa Senol

  2. Departments of Microbiology, Inonu University School of Medicine, Malatya, Turkey

    I. Halil Ozerol

  3. Department of Pediatrics, University of Pittsburgh School of Medicine, Section of Allergy/Immunology, Pittsburgh, PA, USA

    Asha V. Patel & David P. Skoner

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  1. Mustafa Senol
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  2. I. Halil Ozerol
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  3. Asha V. Patel
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  4. David P. Skoner
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Correspondence to Mustafa Senol.

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Senol, M., Ozerol, I.H., Patel, A.V. et al. The effect of Na+-K+ ATPase inhibition by ouabain on histamine release from human cutaneous mast cells. Mol Cell Biochem 294, 25–29 (2007). https://doi.org/10.1007/s11010-006-9180-0

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  • DOI: https://doi.org/10.1007/s11010-006-9180-0

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