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Cloning and characterization of a novel human phosphatidic acid phosphatase type 2, PAP2d, with two different transcripts PAP2d_v1 and PAP2d_v2

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Abstract

This study reports the cloning and characterization of a novel human phosphatidic acid phosphatase type 2 isoform cDNAs (PAP2d) from the foetal brain cDNA library. The PAP2d gene is localized on chromosome 1p21.3. It contains six exons and spans 112 kb of the genomic DNA. By large-scale cDNA sequencing we found two splice variants of PAP2d, PAP2d_v1 and PAP2d_v2. The PAP2d_v1 cDNA is 1722 bp in length and spans an open reading frame from nucleotide 56 to 1021, encoding a 321aa protein. The PAP2d_v2 cDNA is 1707 bp in length encoding a 316aa protein from nucleotide 56–1006. The PAP2d_v1 cDNA is 15 bp longer than the PAP2d_v2 cDNA in the terminal of the fifth exon and it creates different ORF. Both of the proteins contain a well-conserved PAP2 motif. The PAP2d_v1 is mainly expressed in human brain, lung, kidney, testis and colon, while PAP2d_v2 is restricted to human placenta, skeletal muscle, and kidney. The two splice variants are co-expressed only in kidney.

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Authors and Affiliations

  1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, People’s Republic of China

    Liyun Sun, Shaohua Gu, Yaqiong Sun, Dan Zheng, Qihan Wu, Jianfeng Dai, Chaoneng Ji, Yi Xie & Yumin Mao

  2. Shanghai Center for Bioinformation Technology, Shanghai, People’s Republic of China

    Xin Li & Jianliang Dai

  3. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, 200433, People’s Republic of China

    Yumin Mao

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  1. Liyun Sun
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  2. Shaohua Gu
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  3. Yaqiong Sun
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  4. Dan Zheng
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  5. Qihan Wu
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  6. Xin Li
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  7. Jianfeng Dai
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  8. Jianliang Dai
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  9. Chaoneng Ji
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  10. Yi Xie
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  11. Yumin Mao
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Corresponding author

Correspondence to Yumin Mao.

Additional information

The nucleotide sequences reported in this paper have been submitted to GenBank under accession number: AY574039 (PAP2d_v1) and AY634620 (PAP2d_v2).

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Sun, L., Gu, S., Sun, Y. et al. Cloning and characterization of a novel human phosphatidic acid phosphatase type 2, PAP2d, with two different transcripts PAP2d_v1 and PAP2d_v2. Mol Cell Biochem 272, 91–96 (2005). https://doi.org/10.1007/s11010-005-7640-6

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  • DOI: https://doi.org/10.1007/s11010-005-7640-6

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