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Primary Structure of NM.BstSEI Operon from Bacillus stearothermophilus, the Producer of N.BstSEI Site-Specific Nicking Endonuclease

  • Genomics. Transcriptomics. Proteomics
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Abstract

The nucleotide sequence of Bacillus stearothermophilus SE-589 DNA fragment including an operon for the site-specific nicking-modification (NM) system with a gene for BstSEI nicking endonuclease (nickase) has been determined. An analysis of the regions adjacent to the nickase gene has revealed two genes encoding DNA methyltransferases belonging to different classes. Three genes that form the system operon are separated by short open reading frames (ORFs). An analysis of these ORFs has shown that the polypeptides they encode are homologous to different parts of BstSEI nickase, NatB protein, and arginase. A difference in the GC content of the beginning and ending regions of the cloned DNA fragment and the presence of short ORFs similar to genes for known proteins indicate that the NM.BstSEI system operon has probably evolved by horizontal DNA transfer.

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Authors and Affiliations

  1. SibEnzyme Research and Production Association, Novosibirsk, 630117, Russia

    N. S. Gololobova & S. S. Okhapkina

  2. Institute of Molecular Biology and Biophysics, Siberian Division, Russian Academy of Medical Sciences, Novosibirsk, 630117, Russia

    M. A. Abdurashitov & S. Kh. Degtyarev

Authors
  1. N. S. Gololobova
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  2. S. S. Okhapkina
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  3. M. A. Abdurashitov
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  4. S. Kh. Degtyarev
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__________

Translated from Molekulyarnaya Biologiya, Vol. 39, No. 6, 2005, pp. 960–964.

Original Russian Text Copyright © 2005 by Gololobova, Okhapkina, Abdurashitov, Degtyarev.

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Gololobova, N.S., Okhapkina, S.S., Abdurashitov, M.A. et al. Primary Structure of NM.BstSEI Operon from Bacillus stearothermophilus, the Producer of N.BstSEI Site-Specific Nicking Endonuclease. Mol Biol 39, 840–844 (2005). https://doi.org/10.1007/s11008-005-0103-z

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  • DOI: https://doi.org/10.1007/s11008-005-0103-z

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