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Potential Efficacy of Biofilm-Forming Biosurfactant Bacillus firmus HussainT-Lab.66 Against Rhizoctonia solani and Mass Spectrometry Analysis of its Metabolites

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Abstract

Soil-borne pathogens that once persist in the soil cause severe economic losses in agriculture production worldwide including potatoes. Therefore, biological control of plant pathogens assumes greater importance at this juncture. In vitro screening tests were carried out with new potential species of Bacillus firmus HussainT-Lab.66 against black scurf of potato to investigate the biocontrol efficacy. The new biofilm-based bioagent inhibits the growth of Rhizoctonia solani by 87.70% ± 0.20 under the dual culture test. There was a significant positive effect of the new antagonistic bacteria against the pathogen. Through Thin layer chromatography and Fourier transform infrared spectroscopy, the biosurfactant was characterized as lipopeptide further confirming the presence of surfactin through Liquid chromatography-mass spectrometry. A remarkable 71% and 45% reduction in disease incidence under pot and field conditions, respectively, were noticed displaying a remarkable effect of the new bioagent against black scurf of potato tubers. These results indicated that the new bioagent was suppressing the disease incidence. These new bio-agents may be further explored commercially and proteomic techniques can also be applied to investigate the effects on cell membrane proteins of various bacteria.

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Data Availability

All data associated with the work were mentioned in the manuscript.

Abbreviations

TLC:

Thin layer chromatography

FTIR:

Fourier transform infrared spectroscopy analysis

LCMS–ESI:

Liquid chromatography–mass spectrometry

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Acknowledgements

The First Author: Touseef Hussain is very thankful to Start-up Grant by SERB-Dept. of Science and Technology, New Delhi, Govt. of India, for providing the financial grants received under File No. DST-SERB-NPDF/2016/001409 and Dept. of Botany, Aligarh Muslim University, Aligarh, U.P for proving all the support, Dept. of Chemistry, for FT-IR facility.

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  1. Plant Pathology and Nematology Section, Department of Botany, Aligarh Muslim University, Aligarh, 202002, India

    Touseef Hussain & Abrar Ahmad Khan

  2. Biological and Geological Sciences Department, Faculty of Education, Ain Shams University, Cairo, Egypt

    Heba I. Mohamed

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TH: conceptualization, methodology, software. Writing- original draft preparation. Visualization, investigation. Writing- editing, corrections. AAK: supervision, HIM: revising and editing. All authors have read and approved the manuscript and ensure that this is the case.

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Hussain, T., Khan, A.A. & Mohamed, H.I. Potential Efficacy of Biofilm-Forming Biosurfactant Bacillus firmus HussainT-Lab.66 Against Rhizoctonia solani and Mass Spectrometry Analysis of its Metabolites. Int J Pept Res Ther 28, 3 (2022). https://doi.org/10.1007/s10989-021-10318-5

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