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Visfatin Inhibits Apoptosis and Necrosis of Hippocampus CA3 Cells Following Transient Global Ischemia/Reperfusion in Rats

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Abstract

Visfatin, a novel adipokine, is predominantly produced by visceral adipose tissue and it has been linked to a diverse variety of cellular processes and it is an important factor in cell apoptosis and survival. Cerebral ischemia causes loss of hippocampus pyramidal cells. In this study, we investigated the neuroprotective effects of visfatin in rats after global cerebral ischemia. Both common carotid arteries were occluded for 20 min followed by reperfusion. Saline as a vehicle and visfatin at doses of 100 ng were injected intracerebro-ventriculary at the time of cerebral reperfusion. Apoptosis and necrosis were assessed 96 h after ischemia. The results showed that treatment with visfatin significantly reduces apoptosis (P < 0.05) and necrotic cell death (P < 0.001) in hippocampal CA3 area, compared to the ischemia group. In conclusion, visfatin treatment, found for the first time in the present study, reduces hippocampus CA3 injuries after cerebral ischemia through preventing neuronal necrosis and apoptosis.

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Acknowledgments

This research was supported by a Grant (under the contract number 91052159) sponsored by the Iran National Science Foundation (INSF). The authors are very grateful to INSF for financial support. This article does not contain any studies with human or animal subjects performed by any of the authors.

Conflict of interest

Sohaila Erfani, Nahid Aboutaleb, Shahrbanoo Oryan, Nabi Shamsaei, Mehdi Khaksari, Hamid Kalalian-Moghaddam, Farnaz Nikbakht, declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Animal Physiology, Faculty of Biology, Kharazmi University, Tehran, Iran

    Sohaila Erfani & Shahrbanoo Oryan

  2. Physiology Research Center and Department of Physiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran

    Nahid Aboutaleb & Farnaz Nikbakht

  3. Department of Physical Education & Sports Science, Faculty of Literature and Humanities, Ilam University, Ilam, Iran

    Nabi Shamsaei

  4. School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran

    Mehdi Khaksari & Hamid Kalalian-Moghaddam

Authors
  1. Sohaila Erfani
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  2. Nahid Aboutaleb
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  3. Shahrbanoo Oryan
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  4. Nabi Shamsaei
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  5. Mehdi Khaksari
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  6. Hamid Kalalian-Moghaddam
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  7. Farnaz Nikbakht
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Correspondence to Mehdi Khaksari.

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Erfani, S., Aboutaleb, N., Oryan, S. et al. Visfatin Inhibits Apoptosis and Necrosis of Hippocampus CA3 Cells Following Transient Global Ischemia/Reperfusion in Rats. Int J Pept Res Ther 21, 223–228 (2015). https://doi.org/10.1007/s10989-014-9449-1

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  • DOI: https://doi.org/10.1007/s10989-014-9449-1

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