The effect of Haemonchus contortus galectin peptides rHco-gal-m/f to induce apoptosis in the peripheral blood lymphocytes (PBLCs) of goats was investigated. Analysis of apoptosis was carried out with agarose gel electrophoresis, flow cytometry and transmission electron microscopy. The results indicated that there were visible apoptosis bodies and typical DNA ladders by genomic DNA fragmentation. The quantitative analysis of apoptosis by flow cytometry indicated that rHco-gal-m/f peptides induced apoptosis was time and dose dependent. Ultrastructural studies of the PBLCs revealed that a large number of apoptotic cells were present in galectin-treated cells, which had the typical morphologic changes of apoptosis such as reduction of the cytoplasmic volume, loss of cell surface microvilli, chromatin condensation and fragmentation of the apoptotic cells into small apoptotic bodies.
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Abbreviations
- PBLCs:
-
peripheral blood lymphocytes
- PBMCs:
-
peripheral blood monocytes
- rHco-gal-m/f:
-
recombinant galectin of male and female Haemonchus contortus
- CRD:
-
carbohydrate recognition domain
- IL-1β and 4:
-
interleukin-1β and 4
- TNF-α:
-
tumor necrosis factor-α
- IFN-γ:
-
interferon-γ
- PBS:
-
phosphate-buffered saline
- PI:
-
propidium iodide
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Acknowledgments
This work was funded by a grant from the National Natural Science Foundation of P.R. China (No. 30071078). The authors wish to thank Professor Roger K. Prichard for critically reading the manuscript.
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Sun, Y., Yan, R., Muleke, C.I. et al. Recombinant Galectins of Haemonchus contortus Parasite Induces Apoptosis in the Peripheral Blood Lymphocytes of Goat. Int J Pept Res Ther 13, 387–392 (2007). https://doi.org/10.1007/s10989-006-9045-0
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DOI: https://doi.org/10.1007/s10989-006-9045-0