Abstract
Currently, the most examinations and markers are of limited diagnostic and prognostic value in chronic inflammation of the pancreas and its malignant tumorous disease. The purpose of this pilot study was to measure thermal changes of blood plasma by differential scanning calorimetry (DSC) method on patients with chronic pancreatitis, and with operable or inoperable pancreatic adenocarcinoma. The study involved chronic pancreatitis patients (n = 5), in whom had to perform surgery due to any complications. In malignant pancreatic cancer group, according to resectability of the tumors, patients were divided into operable (curative R0 resection, n = 11) and inoperable (palliative double bypass, n = 5) subgroups. Peripheral blood samples were collected from the patients preoperatively and from healthy controls (n = 5). Denaturation of plasma components was detected in Setaram Micro DSC-II calorimeter. DSC results showed decrease of T m1 (48.8 °C) and T m2 (61.8 °C) and increase of T m3 (68 °C) in chronic pancreatitis group compared to healthy controls (56.2, 63.1 and 68 °C). Similar tendencies were in patients with operable (48.2, 61.5, 67.6 °C) and inoperable (48.1, 62.4, 69 °C) pancreas adenocarcinoma. Calorimetric enthalpy mildly decreased in each group except for operable group (1.3 J g−1 ∆H) compared to controls (1.2 J g−1 ∆H). This research confirmed that DSC parameters of blood plasma on patients with chronic pancreatitis and pancreatic adenocarcinoma are clearly distinct from thermodynamical data of healthy controls. After better validation of calorimetric data, it can be a noninvasive tool for diagnostic and monitoring of pancreatic diseases.
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Acknowledgements
The authors especially thank Dezső Kelemen and Róbert Papp, Surgery Clinic, University of Pécs, for surgical inventions and samples availability of pancreas patients. This work was supported by Grants OTKA CO-272 (for Dénes Lőrinczy). The present scientific contribution is dedicated to the 650th anniversary of the foundation of the University of Pécs, Hungary.
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Ferencz, A., Lőrinczy, D. DSC measurements of blood plasma on patients with chronic pancreatitis and operable and inoperable pancreatic adenocarcinoma. J Therm Anal Calorim 127, 1187–1192 (2017). https://doi.org/10.1007/s10973-016-5371-4
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DOI: https://doi.org/10.1007/s10973-016-5371-4