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Structural characterization and thermal behaviour of biological hydroxyapatite

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Abstract

Thermal study and structural characterization of biological hydroxyapatite (HA) samples were done as well as their comparison with commercial and synthetic samples in this study. The X-ray micro analyser shows that all three samples of human teeth (HT1–HT3) contain two types of HA structures with different crystallite sizes, unlike sample of bovine thigh-bone (BTB). The bone sample was composed only of one HA phase with varied porosity. The molar Ca/P ratio in biological samples was lower compared to theoretical ratio for pure HA; moreover, in the case of teeth, Ca/P ratio varyies between the centre and the periphery of the cross-sectional samples. Thermogravimetry of the biological samples showed mass decreases—three regions for the bone and four regions for the teeth. In comparison, commercial HA has only two-step weight loss and synthetic HA three-step weight loss. After the calcination up to 1280 °C all the samples of teeth transformed into whitlockite, β-(Ca,Mg)3(PO4)2 (98 wt%) and 2 wt% HA. Besides, HT3 contained further trace amount of hilgenstockite (HIL, Ca4P2O9). The sample BTB partly transited from natural HA into HIL (6 wt%) and lime, CaO (14 wt%). X-ray powder diffraction (XRD) proved occurrence of HIL (9 wt%) beside stability part HA (91 wt%) in the commercial HA after thermal treatment but the synthetic HA composed from Ca3(PO4)2 (74 wt%) and HA (26 wt%).

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Acknowledgements

This study was supported by the Czech Ministry of Education, Youth and Sports under the project MSM 0021627501, and IGA University of Pardubice (SGFChT04). Special thanks to Milan Vlček from Joint Laboratory of Solid State Chemistry, University of Pardubice.

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Authors and Affiliations

  1. Faculty of Chemical Technology, Department of Inorganic Technology, University of Pardubice, Doubravice 41, 532 10, Pardubice, Czech Republic

    Anna Kohutová, Pavla Honcová & Ladislav Svoboda

  2. Institute of Inorganic Chemistry of the ASCR, v. v. i, Husinec-Řež 1001, 250 68, Řež, Czech Republic

    Petr Bezdička & Monika Maříková

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  1. Anna Kohutová
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  2. Pavla Honcová
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  3. Ladislav Svoboda
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Correspondence to Anna Kohutová or Pavla Honcová.

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Kohutová, A., Honcová, P., Svoboda, L. et al. Structural characterization and thermal behaviour of biological hydroxyapatite. J Therm Anal Calorim 108, 163–170 (2012). https://doi.org/10.1007/s10973-011-1942-6

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  • DOI: https://doi.org/10.1007/s10973-011-1942-6

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