Skip to main content
SpringerLink
Log in

Strategy to enhance catalytic activity and stability of sol–gel oxidoreductases

  • Original Paper: Fundamentals of sol–gel and hybrid materials processing
  • Published:
Journal of Sol-Gel Science and Technology Aims and scope Submit manuscript

Abstract

Oxidoreductases are widely recognized for their capability to degrade phenolic pollutants and versatile. However, the lack of enzyme stability makes this technique unrealistic for industrial applications. In order to enhance their catalytic activity, stability and reusability, oxidoreductases namely laccases and peroxidases were entrapped in sol–gel silica and their catalytic activities were measured by an enzymatic assay using 2,6-dimethoxyphenol and guaiacol as substrates, respectively. The sol–gel silica matrices acted as a polymeric framework around the enzyme is a promising tool for improving enzyme stability. After entrapment, the catalytic activity and stability of sol–gel laccase and peroxidase toward pH, temperature and storage duration remarkably enhanced.

Highlights

  • The enzyme immobilization using novel sol-gel method is a promising method for improving enzyme catalytic activity and stability.

  • After entrapment, the catalytic activity and stability of the immobilized laccase and immobilized peroxidase towards pH, temperature and storage time are significantly improved.

  • Oxidoreductase entrapment in sol-gel silica-based matrix shows a bright future for the use of immobilized enzyme in vast industrial process particularly removal of dye from wastewater treatment.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Georgieva S, Godjevargova T, Mita DG, Diano N, Menale C, Nicolucci C, Romano Carratelli C, Mita L, Golovinsky E (2010) Non-isothermal bioremediation of waters polluted by phenol and some of its derivatives by laccase covalently immobilized on polypropylene membranes. J Mol Catal B 66:210–218

    Article  CAS  Google Scholar 

  2. Majeau J, Brar SK, Tyagi RD (2010) Laccases for removal of recalcitrant and emerging pollutants. Bioresour Technol 101:2331–2350

    Article  CAS  Google Scholar 

  3. Qiu H, Lu L, Huang X, Zhang Z, Qu Y (2010) Immobilization of horseradish peroxidase on nanoporous copper and its potential applications. Bioresour Technol 101:415–9420

    Article  Google Scholar 

  4. Li X, Li S, Liang X, Julian D, McClements, Liu X, Liu F (2020) Applications of oxidases in modification of food molecules and colloidal systems: laccase, peroxidase and tyrosinase. Trends Food Sci Technol 103:78–93

    Article  CAS  Google Scholar 

  5. Debnath R, Saha T (2020) An insight into the production strategies and applications of the ligninolytic enzyme laccase from bacteria and fungi. Biocatal Agric Biotechnol 26:101645

    Article  Google Scholar 

  6. Peralta-Zamora P, Pereira CM, Tiburtius ERL, Moraes SG, Rosa MA, Minussi RC (2003) Decolorization of reactive dyes by immobilized laccase. Appl Catal B 42:131–144

    Article  CAS  Google Scholar 

  7. Mohidem NA, Mat HB (2012) Catalytic activity and stability of laccase entrapped in sol–gel silica with additives. J Sol–Gel Sci Technol 61:96–103

    Article  CAS  Google Scholar 

  8. Mohidem (2018) Biodegradation of alkanolamines in batch and packed-bed reactors using free laccase and sol–gel laccase. Ph.D thesis. Universiti Teknologi Malaysia, Skudai, Malaysia

  9. Wahab RA, Elias N, Abdullah F, Ghoshal SK (2020) On the taught new tricks of enzymes immobilization: an all-inclusive overview. React Funct Polym 152:104613

    Article  CAS  Google Scholar 

  10. Fopase R, Paramasivam S, Kale P, Paramasivan B (2020) Strategies, challenges and opportunities of enzyme immobilization on porous silicon for biosensing applications. J Environ Chem Eng 8(5):104266

    Article  CAS  Google Scholar 

  11. Salis A, Pisano M, Monduzzi M, Solinas V, Sanjust E (2009) Laccase from Pleurotus sajor-caju on functionalised SBA-15 mesoporous silica: Immobilization and use for the oxidation of phenolic compounds. J Mol Catal B Enzym 58:175–180

    Article  CAS  Google Scholar 

  12. Rekuc A, Bryjak J, Szymansja K, Jarzebski AB (2010) Very stable silica–gel-bound laccase biocatalysts for the selective oxidation in continuous systems. Bioresour Technol 101:2076–2083

    Article  CAS  Google Scholar 

  13. Tian F, Xu B, Zhu L, Zhu G (2001) Hydrogen peroxide biosensor with enzyme entrapped within electrodeposited polypyrrole based on mediated sol–gel derived composite carbon electrode. Anal Chim Acta 443(1):9–16

    Article  CAS  Google Scholar 

  14. Yamanaka SA, Nishida F, Ellerby LM, Nishida CR, Dunn B, Valentine JS, Zink J (1992) Enzymatic activity of glucose oxidase encapsulated in transparent glass by the sol–gel method. Chem Mater 4(3):495–497

    Article  CAS  Google Scholar 

  15. Matijosyte I, Arends IWCE, de Vries S, Sheldon RA (2010) Preparation and use of cross-linked enzyme aggregates (CLEAs) of laccase. J Mol Catal B 62:142–148

    Article  CAS  Google Scholar 

  16. Michizoe J, Goto M, Furusaki S (2001) Catalytic activity of laccase hosted in reversed micelles. J Biosci Bioeng 92:67–71

    Article  CAS  Google Scholar 

  17. Vasquez-Duhalt R, Tinoco R, D’Antonio P, Topoleski LDT, Payne GF (2001) Enzyme conjugation to the polysaccharide chitosan: smart biocatalysts and biocatalytic hydrogels. Bioconjugate Chem 12:301–306

    Article  Google Scholar 

  18. Miao Y, Tan SN (2001) Amperometric hydrogen peroxide biosensor with silica sol–gel/chitosan film as immobilization matrix. Anal Chim Acta 437(1):87–93

    Article  CAS  Google Scholar 

  19. Zawisza I, Rogalski J, Opallo M (2006) Electrocatalytic reduction of dioxygen by redox mediator and laccase immobilized in silicate thin film. J Electroanal Chem 588:244–252

    Article  CAS  Google Scholar 

  20. Ellerby LM, Nishida CR, Nishida F, Yamanaka S, Dunn B, Valentine JS, Zink JI (1992) Encapsulation of proteins in transparent porous silicate glasses prepared by the sol–gel method. Science 255(5048):1113–1115

    Article  CAS  Google Scholar 

  21. Gill I, Ballesteros A (1998) Encapsulation of biologicals within silicate, siloxane, and hybrid sol–gel polymers: an efficient and generic approach. J Am Chem Soc 120:8587–8598

    Article  CAS  Google Scholar 

  22. Glad M, Norrlo¨w O, Sellergren B, Siegbahn N, Mosnach K (1985) Use of silane monomers for molecular imprinting and enzyme entrapment in polysiloxane-coated porous silica. J Chromatogr 347:11–23

    Article  CAS  Google Scholar 

  23. Matsune H, Jogasaki H, Date M, Takenaka S, Kishida M (2006) One-pot synthesis and characterization of laccase-entrapped magnetic nanobeads. Chem Lett 35:1356–1357

    Article  CAS  Google Scholar 

  24. Crecchio C, Ruggiero P, Pizzigallo MDR (1995) Polyphenoloxidases immobilized in organic gels: Properties and applications in the detoxification of aromatic compounds. Biotechnol Bioeng 48:585–591

    Article  CAS  Google Scholar 

  25. Okazaki S, Goto M, Wariishi H, Tanaka H, Furusaki S (2000) Characterization and catalytic property of surfactant-laccase complex in organic media. Biotechnol Prog 16:583–588

    Article  CAS  Google Scholar 

  26. Khani Z, Jolivalt C, Cretin M, Tingry S, Innocent C (2006) Alginate/carbon composite beads for laccase and glucose oxidase encapsulation: application in biofuel cell technology. Biotechnol Lett 28:1779–1786

    Article  CAS  Google Scholar 

  27. Shin MY, Park JY, Park K, Song SH, Yoo YJ (2007) Novel sol–gel immobilization of horseradish peroxidase employing a detergentless micro-emulsion system. Biotechnol Bioprocess Eng 12:640–645

    Article  CAS  Google Scholar 

  28. Shakeri M, Shoda M (2010) Efficient decolorization of an anthraquinone dye by recombinant dye-decolorizing peroxidase (rDyP) immobilized in silica-based mesocellular foam. J Mol Catal B 62:277–281

    Article  CAS  Google Scholar 

  29. Mat HB, Mohidem NA (2012) The catalytic activity enhancement and biodegradation potential of free laccase and novel sol–gel laccase in non-conventional solvents. Bioresour Technol 114:472–477

    Article  Google Scholar 

  30. Mohidem NA, Mat HB (2009) The catalytic activity of laccase immobilized in sol–gel silica. J Appl Sci 9:3141–3145

    Article  CAS  Google Scholar 

  31. Vera-Avila LE, Morales-Zamudio E, Garcia-Camacho MK (2004) Activity and reusability of sol–gel encapsulated α-amylase and catalase. Performance in flow-through systems. Sol–Gel Sci Technol 30:197–204

    Article  CAS  Google Scholar 

  32. Leoniwicz A, Sarkar JM, Bollag JM (1988) Improvement fungal laccase. J Chem Biotechnol 29:129–135

    Google Scholar 

  33. Luckarift HR, Ku BS, Dordick JS, Spain JS (2007) Silica-immobilized enzymes for multi-step synthesis in microfluidic devices. Biotechnol Bioeng 98:701–705

    Article  CAS  Google Scholar 

  34. Guisan JM (2006) Immobilization of enzymes and cells. Humana Press, New Jersey

    Book  Google Scholar 

  35. Moniruzzaman M, Nakashima K, Kamiya N, Goto M (2010) Recent advances of enzymatic reactions in ionic liquids. Biochem Eng J 48:295–314

    Article  CAS  Google Scholar 

  36. Bilal M, Asgher M (2016) Enhanced catalytic potentiality of Ganoderma lucidum IBL-05 manganese peroxidase immobilized on sol–gel matrix. J Mol Catal B: Enzym 128:82–93

    Article  CAS  Google Scholar 

  37. Nawawi NN, Hashim Z, Rahman RA, Murad AMA, Bakar FDA, Illias RM (2020) Entrapment of porous cross-linked enzyme aggregates of maltogenicamylase from Bacillus lehensisG1 into calcium alginate formaltooligosaccharides synthesis. Int J Biol Macromol 150:80–89

    Article  CAS  Google Scholar 

  38. Das S, Berke-Schlessel D, Ji H-F, McDonough J, Wei Y (2011) Enzymatic hydrolysis of biomass with recyclable use of cellobiase enzymeimmobilized in sol–gel routed mesoporous silica. J Mol Catal B: Enzym 70:49–54

    Article  CAS  Google Scholar 

  39. Braun S, Rappoport S, Zusman R, Avnir D, Ottolenghi M (2007) Biochemically active sol–gel glasses: The trapping of enzymes. Mater Lett 61:2843–2846

    Article  CAS  Google Scholar 

  40. Yi Y, Neufeld R, Kermasha S (2007) Controlling sol–gel properties enhancing entrapped membrane protein activity through doping additives. Sol–Gel Sci Technol 43:161–170

    Article  CAS  Google Scholar 

  41. Fernandez-Lafuente R (2009) Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation. Enzyme Microb Technol 45:405–418

    Article  CAS  Google Scholar 

  42. Bhatia RB, Brinker CJ (2000) Aqueous sol–gel process for protein encapsulation. Chem Mater 12:2434–2441

    Article  CAS  Google Scholar 

  43. Makas YG, Kalkan NA, Aksoy S, Altinok H, Hasirci N (2010) Immobilization of laccase in κ-carrageenan based semi-interpenetrating polymer networks. J Biotechnol 148:216–220

    Article  CAS  Google Scholar 

  44. Arica MY, Bayramoglu G, Bicak N (2004) Characterization of tyrosinase immobilized onto spacer-arm attached glycidyl methacrylate-based reactive microbeads. Process Biochem 39:2007–2017

    Article  CAS  Google Scholar 

  45. Chen JP, Lin WS (2003) Sol–gel powders and supported sol–gel polymers for immobilization of lipase in ester synthesis. Enzyme Microb Technol 32:801–811

    Article  CAS  Google Scholar 

  46. Creighton TE (1996) Proteins structure and molecular properties. WH Freeman and Company, New York, NY

    Google Scholar 

  47. Shuler ML, Kargi F (2005) Bioprocess engineering. Prentice Hall, Upper Saddle River, New Jersey

    Google Scholar 

  48. Mohidem NA, Mansor AF, Wan Mohd Zawawi WNI, Othman NS, Mat HB (2016) Effect of synthesis conditions on physical properties, catalytic activity and stability of sol–gel laccase. J Sol–Gel Sci Technol 80:587–597

    Article  CAS  Google Scholar 

  49. Ahmad Shamsuri NA, Mohidem NA, Mansor AF, Mat HB (2012) Biodegradation of dye using free laccase and sol–gel laccase. J Teknol 59:39–42

    Article  Google Scholar 

  50. Aegerter MA, Mennig M (2004) Sol–gel technologies for glass producers and users. Springer, Boston MA

    Book  Google Scholar 

Download references

Acknowledgements

The authors would like to acknowledge the financial support from the Ministry of Higher Education (MOHE), Malaysia under the Fundamental Research Grant Scheme (FRGS Project No. 78213).

Author information

Authors and Affiliations

  1. Advanced Materials and Process Engineering (AMPEN) Research Group, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, 81310 UTM, Skudai, Johor, Malaysia

    Nur Atikah Mohidem & Hanapi Bin Mat

  2. Faculty of Bioengineering and Technology, Universiti Malaysia Kelantan, Jeli Campus, 17600, Jeli, Kelantan, Malaysia

    Mardawani Mohamad

  3. Faculty of Chemical Engineering, Universiti Teknologi MARA, 40450, Shah Alam, Selangor, Malaysia

    Fazlena Hamzah

  4. Institute of Chemical Engineering & Technology, University of the Punjab, Lahore, Pakistan

    Muhammad Usman Rashid

Authors
  1. Nur Atikah Mohidem
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hanapi Bin Mat
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mardawani Mohamad
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fazlena Hamzah
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Muhammad Usman Rashid
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Nur Atikah Mohidem.

Ethics declarations

Conflict of interest

The authors declare no competing interests.

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mohidem, N.A., Bin Mat, H., Mohamad, M. et al. Strategy to enhance catalytic activity and stability of sol–gel oxidoreductases. J Sol-Gel Sci Technol 98, 462–469 (2021). https://doi.org/10.1007/s10971-021-05522-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10971-021-05522-0

Keywords

Search

Navigation