Skip to main content
SpringerLink
Log in

Alginate/carbon composite beads for laccase and glucose oxidase encapsulation: application in biofuel cell technology

  • Origianl Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Alginate–carbon beads were prepared in order to develop a biocompatible matrix for laccase and glucose oxidase immobilization for application in biofuel cell technology. The enzyme loading capacity was high (91%) in pure alginate beads for glucose oxidase. For laccase, the loading capacity was enhanced from 75% to 83% by introducing carbon. Desorption out of the matrix was controlled by the enzymes’ diffusion and reached a plateau after 40 h for laccase and 70 h for glucose oxidase. Two-thirds of both enzymes was irreversibly retained inside the alginate beads. This proportion increased to 80% for laccase in combined alginate/carbon beads. Half-life of the adsorbed enzyme was enhanced to 74 days for laccase in carbon/alginate beads and 45 days for glucose oxidase in pure alginate as compared to 38 days and 23 days for free enzymes, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ates S, Mehmetoglu U (1997) A new method for immobilization of β-galactosidase and its utilization in a plug flow reactor. Proc Biochem 32(5):433–436

    Article  CAS  Google Scholar 

  • Balcao VM, Paivra AL, Malcata FX (1996) Bioreactors with immobilized lipases: state of the art. Enzyme Microb Technol 18:392–416

    Article  PubMed  CAS  Google Scholar 

  • Barton SC, Gallaway J, Atanassov P (2004) Enzymatic biofuel cells for implantable and microscale devices. Chem Rev 104:4867–4886

    Article  PubMed  CAS  Google Scholar 

  • Bertrand T, Jolivalt C, Briozzo P, Caminade E, Joly N, Madzak C, Mougin C (2002) Crystal structure of a four-copper laccase complexed with an arylamine: insights into substrate recognition and correlation with kinetics. Biochemistry 41:7325–7333

    Article  PubMed  CAS  Google Scholar 

  • Birnbaum (1992) Immobilisation of macromolecules and cells. In: Sletz UB, Messner P, Pum D, Sara M (eds) Immobilised macromolecules. Springer-Verlag, pp 23–35

  • Blandino A, Macias M, Cantero D (1999) Formation of calcium alginate capsules: influence of sodium alginate and CaCl2 concentration on gelation kinetics. J Biosci Bioeng 88(6):686–689

    Article  PubMed  CAS  Google Scholar 

  • Blandino A, Macias M, Cantero D (2000) Glucose oxidase release from calcium alginate gel capsules. Enzyme Microb Technol 27:319–324

    Article  PubMed  CAS  Google Scholar 

  • Blandino A, Macias M, Cantero D (2001) Immobilization of glucose oxidase within calcium alginate gel capsules. Process Biochem 36:601–606

    Article  CAS  Google Scholar 

  • Bulmus V, Ayhan H, Piskin E (1997) Modified PMMA monosize microbeads for glucose oxidase immobilization. Chem Eng J 65:71–76

    Article  CAS  Google Scholar 

  • Catana R, Ferreira BS, Cabral JMS, Fernandes P (2005) Immobilization of inulinase for sucrose hydrolysis. Food Chem 91:517–520

    Article  CAS  Google Scholar 

  • Coradin T, Nassif N, Livage J (2003) Silica–alginate composites for microencapsulation. Appl Microbiol Biotechnol 61:429–434

    PubMed  CAS  Google Scholar 

  • Dashevsky A (1998) Protein loss by the microencapsulation of an enzyme (lactase) in alginate beads. Int J Pharm 161:1–5

    Article  CAS  Google Scholar 

  • Hari PR, Chandy T, Sharma CP (1996) Chitosan/calcium–alginate beads for oral delivery of insulin. J Appl Polym Sci 59:1795–1801

    Article  CAS  Google Scholar 

  • Jönsson L, Johansson T, Sjöström K, Nyman PO (1987) Purification of ligninase isoenzymes from the white-rot fungus Trametes versicolor. Acta Chem Scand Ser B 41:766–769

    Article  Google Scholar 

  • Jungbae K, Hongfei J, Ping W (2006) Challenges in biocatalysis for enzyme-based biofuel cells. Biotechnol Adv 24:296–308

    Article  CAS  Google Scholar 

  • Katz E (2000) Glucose oxidase (GOx) E.C. 1.1.3.4. Available on the web http://chem.ch.huji.ac.il/∼eugeniik/glucose_oxidase.htm

  • Lesage-Meesen L, Delattre M, Haon M, Thibault JF, Colonna-Ceccaldi B, Brunerie P, Asther M (1996) A two-step bioconversion process for vanillin production from ferulic acidcombining Aspergillus Niger and Pycnoporus cinnabarinus. J Biotechnol 50:107–113

    Article  Google Scholar 

  • Lim F, Sun A (1980) Microencapsulated islets as bioartificial endocrine pancreas. Science 210:908–910

    Article  PubMed  CAS  Google Scholar 

  • Miyazaki S, Nakayama A, Oda M, Takada M, Attwood D (1994) Chitosan and sodium alginate based bioadhesive tablets for intraoral drug delivery. Biol Pharm Bull 17(5):745–747

    PubMed  CAS  Google Scholar 

  • Ottolina G, Carrea G, Riva S, Satore L, Veronese FM (1992) Effect of the enzyme form on the activity, stability and enantioselectivity of lipoprotein lipase in toluene. Biotechnol Lett 14:947–952

    Article  CAS  Google Scholar 

  • Ribeiro CC, Barrias CC, Barbosa MA (2004) Calcium phosphate–alginate microspheres as enzyme delivery matrices. Biomaterials 25:4363–4373

    Article  PubMed  CAS  Google Scholar 

  • Servat K, Tingry S, Brunel L, Querelle S, Cretin M, Innocent C, Jolivalt C, Rolland M (2006) Modification of porous carbon tubes with enzymes: application for biofuel cells. J Appl Electrochem (special issue 7th EEE Toulouse) submitted

  • Tanaka H, Matsumura M, Veliky IA (1984) Diffusion characteristics of substrates in Ca–alginate gel beads. Biotechnol Bioeng 16:53–58

    Article  Google Scholar 

  • Tanriseven A, Dogan S (2001) Immobilization of invertase within calcium alginate gel capsules. Process Biochem 36:1081–1083

    Article  CAS  Google Scholar 

  • Taqieddin E, Amiji M (2004) Enzyme immobilization in novel alginate–chitosan core-shell microcapsules. Biomaterials 25:1937–1945

    Article  PubMed  CAS  Google Scholar 

  • Zeniek J (1992) Introduction to enzyme engineering. In: Gemeiner P (ed) Enzyme engineering. Immobilzed Biosystems. Ellis Horwood, New York, pp 9–12

    Google Scholar 

Download references

Acknowledgement

This research was supported in part by an ACI “Jeunes Chercheurs”—Ministère de la Recherche.

Author information

Authors and Affiliations

  1. Institut Européen des Membranes, UMR 5635, Place Eugène Bataillon, CC 047, 34293, Montpellier Cedex 5, France

    Zoreh Khani, Marc Cretin, Sophie Tingry & Christophe Innocent

  2. Laboratoire de Synthèse Sélective Organique et Produits Naturels UMR 7573, ENSCP, 11 rue Pierre et Marie Curie, 75231, Paris Cedex 05, France

    Claude Jolivalt

Authors
  1. Zoreh Khani
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Claude Jolivalt
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Marc Cretin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sophie Tingry
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Christophe Innocent
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Marc Cretin.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Khani, Z., Jolivalt, C., Cretin, M. et al. Alginate/carbon composite beads for laccase and glucose oxidase encapsulation: application in biofuel cell technology. Biotechnol Lett 28, 1779–1786 (2006). https://doi.org/10.1007/s10529-006-9160-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-006-9160-1

Keywords

Search

Navigation