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Determination of peptide content and purity of DOTA-peptides by metal ion titration and UPLC: an alternative method to monitor quality of DOTA-peptides

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Abstract

PRRT requires high specific activities, thus at low molar ratio between DOTA-peptide and radioactivity. Therefore, the ingredients of the reaction, including (radio)metals and DOTA-peptide must be pure and the content known. Our aim was to quantify content and purity of DOTA-peptide by a base-to-base separation of DOTA-peptide and metal-DOTA-peptide by UPLC and UV-detection. Quantification of these peaks reveals an accurate and sensitive method to quantify purity and content of DOTA-peptides. Moreover, this technique enables monitoring of the (radio)labeling process and co-introduction of impurities, including metal ions.

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Acknowledgments

Dr. Jack L. Erion, BioSynthema, St Louis, MO, USA for fruitful discussions. Eldert Valk and Martin Appeljan are acknowledged for their technical assistance.

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Authors and Affiliations

  1. Department of Nuclear Medicine, Erasmus MC Rotterdam, ‘s Gravendijkwal 230, 3015 CE, Rotterdam, The Netherlands

    Wouter A. P. Breeman, Ho Sze Chan & Erik de Blois

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  1. Wouter A. P. Breeman
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  2. Ho Sze Chan
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  3. Erik de Blois
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Correspondence to Wouter A. P. Breeman.

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Breeman, W.A.P., Chan, H.S. & de Blois, E. Determination of peptide content and purity of DOTA-peptides by metal ion titration and UPLC: an alternative method to monitor quality of DOTA-peptides. J Radioanal Nucl Chem 302, 825–830 (2014). https://doi.org/10.1007/s10967-014-3248-1

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  • DOI: https://doi.org/10.1007/s10967-014-3248-1

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