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Identification of a Lysine Residue Important for the Catalytic Activity of Yeast Farnesyl Diphosphate Synthase

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Abstract

The Saccharomyces cerevisiae ERG20 gene (encoding farnesyl diphosphate synthase) has been subjected to a set of mutations at the catalytic site, at position K254 to determine the in vivo impact. The mutated strains have been shown to exhibit various growth rates, sterol profiles and monoterpenol producing capacities. The results obtained suggest that K at position 254 helps to stabilize one of the three Mg2+ forming a bridge between the enzyme and DMAPP, and demonstrate that destabilizing two of the three Mg2+ ions, by introducing a double mutation at positions K197 and K254, results in a loss of FPPS activity and a lethal phenotype.

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Abbreviations

FPPS:

Farnesyl diphosphate synthase

FPP:

Farnesyl diphosphate

IPP:

Isopentenyl diphosphate

DMAPP:

Dimethylallyl diphosphate

GPP:

Geranyl diphosphate

GGPP:

Geranyl geranyl diphosphate

FARM:

First aspartate rich motif

SARM:

Second aspartate rich motif

GC–MS:

Gas chromatography-mass spectroscopy

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Authors and Affiliations

  1. Laboratoire Métabolisme Secondaire de la Vigne, Univ. Strasbourg, INRA, Inst. Natl. Recherche Agron., Métab. Second. Vigne, Unit Mixte Recherche Santé Vigne and Qual. Vins, 28 rue de Herrlisheim, 68021, Colmar, France

    Marc J. C. Fischer, Sophie Meyer, Patricia Claudel & Francis Karst

  2. The Inst. Biol. Mol. Plantes, Univ Strasbourg, CNRS, 67084, Strasbourg Cedex, France

    Marc Bergdoll

Authors
  1. Marc J. C. Fischer
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  2. Sophie Meyer
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  3. Patricia Claudel
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  4. Marc Bergdoll
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  5. Francis Karst
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Corresponding author

Correspondence to Marc J. C. Fischer.

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Fischer, M.J.C., Meyer, S., Claudel, P. et al. Identification of a Lysine Residue Important for the Catalytic Activity of Yeast Farnesyl Diphosphate Synthase. Protein J 30, 334–339 (2011). https://doi.org/10.1007/s10930-011-9336-y

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  • DOI: https://doi.org/10.1007/s10930-011-9336-y

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