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Amino Acids from Mytilus galloprovincialis (L.) and Rapana venosa Molluscs Accelerate Skin Wounds Healing via Enhancement of Dermal and Epidermal Neoformation

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Abstract

Wound healing consists of re-epithelialization, contraction and formation of granulation and scar tissue. Amino acids from proteins are involved in these events, but their exact roles are not well understood. The present study was undertaken to investigate the anti-inflammatory effects of some amino acids from two molluscs, Mytilus galloprovincialis (L.) (Mediterranean mussel) and Rapana venosa (hard shell-clam) employed in induced skin burn injuries in Wistar rats. The treatment was evaluated in terms of essential amino acids composition which rendered the extracts very efficient in healing skin burns. The healing process was examined by periodic acid Schiff’s, Verhoeff’s Van Gieson and immunohistochemistry stains for collagen IV, CD 34 and CD 117 antibodies. According to the obtained results, as expressed by histological studies, the most abundant blood vessels, collagen fibres, basal and stem cells were found only for treated animals with amino acids from Rapana venosa extracts. The rich composition of amino acids from the two molluscs merits consideration as therapeutic agents in the treatment of skin burns.

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Abbreviations

Ser:

Serine

Ala:

Alanine

Gly:

Glycine

Pro:

Proline

Val:

Valine

Leu:

Leucine

Ile:

Isoleucine

Thr:

Threonine

Met:

Methionine

Lys:

Lysine

Phe:

Phenylalanine

Tyr:

Tyrosine

Trp:

Tryptofan

Asp:

Aspartic acid

Glu:

Glumatic acid

Hypro:

Hydroxyproline

GC–MS:

Gas Chromatography-Mass Spectrometry

CD:

Cluster designation

NBF:

Neutral buffered formalin

DAB:

Diaminobenzidine

HRP:

Horseradish peroxidise

LPS:

Lipopolysaccharide

i.p.:

Intraperitoneal

H2O2 :

Hydrogen peroxide

K2SO4 :

Potassium sulphate

CuSO4 :

Copper sulphate

H2SO4 :

Sulphuric acid

KOH:

Potassium hydroxide

NaCl:

Sodium chloride

Na2SO4 :

Sodium sulphate

HIO4 :

Periodic acid

FeCl3 :

Ferric chloride

O2 :

Oxygen

N2 :

Nitrogen

I:

Iodine

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Authors and Affiliations

  1. Department of Biology-Biochemistry, Faculty of Natural and Agricultural Sciences, Ovidius University of Constanta, 124 Mamaia Blvd., 900527, Constanta, Romania

    Diana L. Badiu

  2. Departament of Organic Chemistry, Cordoba University, Campus de Rabanales, Edificio Marie Curie (C-3), Ctra Nnal IV, Km 396, 14004, Cordoba, Spain

    Rafael Luque

  3. National Institute of Recovery, Physical Medicine and Clinical Balneary, 11 A, Ion Mihalache Blvd., Bucharest, Romania

    Elena Dumitrescu

  4. Phatological Anatomy Laboratory, Constanta Clinical Emergency Hospital, 145 Tomis Blvd, 900591, Constanta, Romania

    Anca Craciun

  5. Otorhinolaryngology Department, Constanta Clinical Emergency Hospital, 145 Tomis Blvd., 900591, Constanta, Romania

    Danut Dinca

Authors
  1. Diana L. Badiu
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  2. Rafael Luque
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  3. Elena Dumitrescu
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  4. Anca Craciun
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  5. Danut Dinca
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Correspondence to Diana L. Badiu.

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Badiu, D.L., Luque, R., Dumitrescu, E. et al. Amino Acids from Mytilus galloprovincialis (L.) and Rapana venosa Molluscs Accelerate Skin Wounds Healing via Enhancement of Dermal and Epidermal Neoformation. Protein J 29, 81–92 (2010). https://doi.org/10.1007/s10930-009-9225-9

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  • DOI: https://doi.org/10.1007/s10930-009-9225-9

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