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Skin Wound Healing Potential and Antioxidant Effect of Hyaluronic Acid Extracted from Mytilus galloprovincialis and Crassostrea gigas

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Pharmaceutical Chemistry Journal Aims and scope

This research work was devoted to studying the ability of hyaluronic acid (HA) to treat skin wounds in rats. HA, an immune-neutral polysaccharide, is widespread in the human body as it is necessary for several tissues and cellular functions and has been used in the clinical practice for more than three decades. For this purpose, we attempted to obtain and enrich HA-containing extracts from two species of molluscs collected at Bizerte lagoon, namely Mytilus galloprovincialis and Crassostrea gigas. Then, the wound healing potential of purified HA (hyaluronan) was tested on Wistar rats which were separated into 4 groups: control, treated with a marketed healing cream “Avene Cicalfate”, treated with HA isolated from M. galloprovincialis, and treated with HA isolated from C. gigas. After anesthesia, rats were submitted to back skin lesions (diameter 2 cm) and then treated daily until recovery. During the experiment, variation of the wound area was monitored. Results showed that the obtained net yield of HA was 3.8 mg/g dry weight and 1.9 mg/g dry weight for M. galloprovincialis and C. gigas respectively. Morphological analysis demonstrated that HA significantly accelerated wound repair through reepithelization. The mean wound surface was completely absent after 15 days of treatment with C. gigas hyaluronan. Based on these findings, it appears that mollusc HA has an obvious healing activity completely in accordance with its recognized effectiveness in dermo-cosmetology.

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Conflict of Interest

The authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Ecology, Biology and Physiology Laboratory of Aquatic Organisms, Biology Department, Faculty of Sciences of Tunis, University Tunis el Manar, 2092, Tunis, Tunisia

    Nawzet Bouriga & Monia Trabelsi

  2. University of Carthage, Higher Institute of Fisheries and Aquaculture of Bizerte, Research Unit: Exploitation of Aquatic Environments, Er-Rimel, B.P.15. 7080, Bizerte, Tunisia

    Nawzet Bouriga & Sami Mili

  3. Laboratory of Biodiversity, Biotechnology and Climate change LR11ES09, Faculty of Sciences of Tunis, University Tunis el Manar, 2092, Tunis, Tunisia

    Wafa Rjiba Bahri

  4. LR13AGR02: ADIPARA Lab, Higher School of Agriculture of Mateur, BP 7030, University of Carthage, 7021, Jarzouna, Tunisia

    Bayrem Jammeli

  5. Laboratory of Biosurveillance of the Environment, Faculty of Science of Bizerte, University of Carthage, 7021, Jarzouna, Tunisia

    Mossadok Ben-Attia

  6. Ichtyology Laboratory, University of Montpellier II, Sciences et Techniques Languedoc, Place Eugene Bataillon, Case 102, 34095, Montpellier, Cedex 5, France

    Jean-Pierre Quignard

Authors
  1. Nawzet Bouriga
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  2. Sami Mili
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  3. Wafa Rjiba Bahri
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  4. Bayrem Jammeli
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  5. Mossadok Ben-Attia
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  6. Jean-Pierre Quignard
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  7. Monia Trabelsi
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Correspondence to Nawzet Bouriga.

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Bouriga, N., Mili, S., Bahri, W.R. et al. Skin Wound Healing Potential and Antioxidant Effect of Hyaluronic Acid Extracted from Mytilus galloprovincialis and Crassostrea gigas. Pharm Chem J 56, 381–386 (2022). https://doi.org/10.1007/s11094-022-02647-6

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  • DOI: https://doi.org/10.1007/s11094-022-02647-6

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