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Kiwellin, a Novel Protein from Kiwi Fruit. Purification, Biochemical Characterization and Identification as an Allergen*

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Abstract

Kiwellin is a novel protein of 28 kDa isolated from kiwi (Actinidia chinensis) fruit. It is one of the three most abundant proteins present in the edible part of this fruit. Kiwellin has been purified by ion exchange chromatography. Its N-terminal amino acid sequence revealed high identity with that previously reported for a 28 kDa protein described as one of the most important kiwi allergens. This observation prompted us to fully characterize this protein. The complete primary structure, elucidated by direct sequencing, indicated that kiwellin is a cysteine-rich protein. Serological tests and Western Blotting analysis showed that kiwellin is specifically recognized by IgE of patients allergic to kiwi fruit.

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Abbreviations

FPLC:

fast protein liquid chromatography

HPLC:

high-performance liquid chromatography

SDS-PAGE:

sodium dodecylsulphate-polyacrylamide gel electrophoresis.

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Authors and Affiliations

  1. Institute of Protein Biochemistry, C.N.R., Via Pietro Castellino 111, I-80131, Napoli, Italy

    Maurizio Tamburrini, Ivana Cerasuolo, Vito Carratore, Laura Camardella & M. Antonietta Ciardiello

  2. Clinica Malattie Respiratorie, University Federico II, Azienda Ospedaliera di Rilievo Nazionale e Alta Specializzazione “V. Monaldi”, Via Leonardo Bianchi, I-80131, Napoli, Italy

    Anna Agnese Stanziola

  3. Laboratori di Analisi – Azienda Ospedaliera di Rilievo Nazionale e Alta Specializzazione “V. Monaldi”, Via Leonardo Bianchi, I-80131, Napoli, Italy

    Sergio Zofra & Luigi Romano

Authors
  1. Maurizio Tamburrini
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  2. Ivana Cerasuolo
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  3. Vito Carratore
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  4. Anna Agnese Stanziola
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  5. Sergio Zofra
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  6. Luigi Romano
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  7. Laura Camardella
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  8. M. Antonietta Ciardiello
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Corresponding author

Correspondence to Maurizio Tamburrini.

Additional information

*The protein sequence data reported in this paper will appear in the Swiss-Prot and TrEMBL knowledgebase under the accessionnumber P84527.

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Tamburrini, M., Cerasuolo, I., Carratore, V. et al. Kiwellin, a Novel Protein from Kiwi Fruit. Purification, Biochemical Characterization and Identification as an Allergen*. Protein J 24, 423–429 (2005). https://doi.org/10.1007/s10930-005-7638-7

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  • DOI: https://doi.org/10.1007/s10930-005-7638-7

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