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Utilizing of Sugar Refinery Waste (Cane Molasses) for Production of Bio-Plastic Under Submerged Fermentation Process

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Abstract

In the present study, depending upon the availability and cheaper cost, different carbon source were tested for the production of PHAs (Polyhydroxyalkonoates) by soil bacterium Pseudomonas aeruginosa and it was found that sugar refinery waste (cane molasses) produced the maximum PHA (biodegradable polymer) which is precursor for bio-plastic development. Urea served as potent nitrogen source over other inorganic nitrogen sources in bio-plastic synthesis. Effect of different physical parameters viz; pH, temperature and agitation speed were also studied on PHA production. Batch cultivation kinetics under optimized cultural and physical condition showed maximum cell mass and PHA concentration of 7.32 ± 0.2 and 5.60 ± 0.3 g/L, respectively after 54.0 h of cultivation. Sugar refinery waste (Total sugar 4%) and urea (0.8%) improved the economics of the process which exhibited a yield (YP/X) of 0.70 with productivity of 0.11 g/L/h. PHA was further characterized as PHB by using Fourier Transform Infra-red Spectroscopy (FT-IR).

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Acknowledgments

We would like to pay our Sincere thanks to Dr. S. B. Roy for providing us FT-IR facility at Department of Physics, Banaras Hindu University, Varanasi-221005, UP, India.

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Authors and Affiliations

  1. Centre of Food Science and Technology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, 221005, India

    Abhishek Dutt Tripathi, Ajay Yadav & Alok Jha

  2. School of Biochemical Engineering, Institute of Technology, Banaras Hindu University, Varanasi, 221005, India

    S. K. Srivastava

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  1. Abhishek Dutt Tripathi
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  2. Ajay Yadav
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  4. S. K. Srivastava
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Correspondence to Abhishek Dutt Tripathi.

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Tripathi, A.D., Yadav, A., Jha, A. et al. Utilizing of Sugar Refinery Waste (Cane Molasses) for Production of Bio-Plastic Under Submerged Fermentation Process. J Polym Environ 20, 446–453 (2012). https://doi.org/10.1007/s10924-011-0394-1

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