Abstract
We identified a biodegrading microorganism of polyamide (nylon) 4, a linear polymer of γ-aminobutyric acid (GABA). From activated sludge, the biodegrading bacteria strains of Pseudomonas sp. were isolated and identified by their taxonomic characteristics and nucleotide sequences of 16S rDNA. One strain, ND-11, was grown on a minimal medium containing polyamide 4 (PA4) as the sole carbon source. The strain produced GABA as a degradation intermediate, as identified by analyzing the NMR spectra of degraded products. The culture supernatant of strain ND-11 degraded the emulsified PA4 completely within one day. These results suggest that the ND-11 strain degraded PA4 using its extracellular enzymes to hydrolyze amide bonds.
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Yamano, N., Nakayama, A., Kawasaki, N. et al. Mechanism and Characterization of Polyamide 4 Degradation by Pseudomonas sp.. J Polym Environ 16, 141–146 (2008). https://doi.org/10.1007/s10924-008-0090-y
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DOI: https://doi.org/10.1007/s10924-008-0090-y