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Single-substrate enzyme kinetics: the quasi-steady-state approximation and beyond

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Abstract

We analyze the standard model of enzyme-catalyzed reactions at various substrate-enzyme ratios by adopting a different scaling scheme and computational procedure. The regions of validity of the quasi-steady-state approximation are noted. Certain prevalent conditions are checked and compared against the actual findings. Efficacies of a few other measures, obtained from the present work, are highlighted. Some recent observations are rationalized, particularly at moderate and high enzyme concentrations.

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Acknowledgments

SD is thankful to CSIR, India for a research fellowship.

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Authors and Affiliations

  1. Department of Chemistry, University of Calcutta, Kolkata, 700 009, India

    Sharmistha Dhatt & Kamal Bhattacharyya

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  1. Sharmistha Dhatt
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  2. Kamal Bhattacharyya
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Correspondence to Kamal Bhattacharyya.

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Dhatt, S., Bhattacharyya, K. Single-substrate enzyme kinetics: the quasi-steady-state approximation and beyond. J Math Chem 51, 1467–1477 (2013). https://doi.org/10.1007/s10910-013-0160-9

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  • DOI: https://doi.org/10.1007/s10910-013-0160-9

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