Abstract
A honeybee colony is a superorganism that has evolved precise communication systems, which allow the colony to gather information from numerous individuals and coordinate its behavior. Alarm pheromones, such as isopentyl acetate (IPA), the main component of sting alarm pheromone, play a critical role in the coordination of individual behaviors as well as colony communication in honeybee colonies. In this study, honeybees (Apis mellifera ligustica and Apis cerana cerana) were exposed to relatively high levels of IPA at a foraging site (6–8 bee equivalents) and inside their colony (28–58 bee equivalents) to investigate the influence of alarm pheromones on foraging activity and hive flight activity. IPA reduced the number of bees that flew out the hive, foraged, and waggle danced. Under both contexts in the hive and at the food source, IPA can therefore inhibit honey bee foraging and foraging communication.
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References
Allan S, Slessor K, Winston M, King G (1987) The influence of age and task specialization on the production and perception of honey bee pheromones. J Insect Physiol 33(12):917–922
Blum MS, Fales HM (1988) Chemical releasers of alarm behavior in the honey bee: informational 'plethora' of the sting apparatus signal. In: Needham GR et al (eds) Africanized honey bees and bee mites. Ellis Horwood Limited, Chichester, UK, p 141–148
Boch R, Shearer DA, Stone BC (1962) Identification of isoamyl acetate as an active component in the sting pheromone of the honey bee. Nature 195(4845):1018–1020
Bowden RM, Garry MF, Breed MD (1994) Discrimination of con-and heterospecific bees by Trigona (Tetragonisca) angustula guards. J Kansas Entomol Soc 67(1):137–139
Breed MD (1991) Defensive behavior. See Ref 127(3):299–308
Breed MD, Guzman-Novoa E, Hunt GJ (2004) Defensive behavior of honey bees: organization, genetics, and comparisons with other bees. Annu Rev Entomol 49:271–298
Collins AM (1981) Effects of temperature and humidity on honeybee response to alarm pheromones. J Apic Res 20:13–18
Collins AM, Blum MS (1983) Alarm responses caused by newly identified compounds derived from the honeybee sting. J Chem Ecol 9(1):57–65
Drum NH, Rothenbuhler WC (1984) Effect of temperature on non-stinging aggressive responses of worker honeybees to diseased and healthy bees. J Apic Res 23:82–87
Dyer FC (2002) The biology of the dance language. Annu Rev Entomol 47:917–949
Free J (1961) The stimuli releasing the stinging response of honeybees. Anim Behav 9(3):193–196
Free JB, Pickett JA, Ferguson AW, Simpkins JR, Smith MC (1985) Repelling foraging honeybees with alarm pheromones. J Agric Sci 105(2):255–260
Frisch KV (1965) Tanzsprache und Orientierung der Bienen. Exp Physiol 51(4):382
Frisch KV (1967) The dance language and orientation of bees. Harvard University Press, Cambridge
Ghent RL, Gary NE (1962) A chemical alarm releaser in honey bee stings (Apis mellifera L.) Psyche 69(1):1–6
Goodale E, Nieh JC (2012) Public use of olfactory information associated with predation in two species of social bees. Anim Behav 84(4):919–924
Gross L (2007) A new view of the waggle dance: making scents to recruit fellow foragers. PLoS Biol 5(9):e249
Hunt GJ (2007) Flight and fight: a comparative view of the neurophysiology and genetics of honey bee defensive behavior. J Insect Physiol 53(5):399–410
Kastberger G, Thenius R, Stabentheiner A, Hepburn R (2009) Aggressive and docile colony defence patterns in apis mellifera. A retreater–releaser concept. J Insect Behav 22:65–85
Kirchner WH (1993) Vibrational signals in the tremble dance of the honeybee, Apis mellifera. Behav Ecol Sociobiol 33(3):169–172
Koeniger N, Weiss J, Maschwitz U (1979) Alarm pheromones of the sting in the genus< i> Apis</i>. J Insect Physiol 25(6):467–476
Li JJ, Wang ZW, Tan K, Qu YF, Nieh JC (2014) Effects of natural and synthetic alarm pheromone and individual pheromone components on foraging behavior of the giant Asian honey bee, Apis dorsata. J Exp Biol 217(1):3512–3518
Nieh JC (1993) The stop signal of honey bees: reconsidering its message. Behav Ecol Sociobiol 33(1):51–56
Nieh JC (2010) A negative feedback signal that is triggered by peril curbs honey bee recruitment. Curr Biol 20(4):310–315
Oldroyd BP, Wongsiri S (2009) Asian honey bees: biology, conservation and human interactions. Harvard University Press, Cambridge
Pankiw T (2004) Cued in: honey bee pheromones as information flow and collective decision-making. Apidologie 35:217–226
Pastor KA, Seeley TD (2005) The brief piping signal of the honey bee: begging call or stop signal? Ethology 111(8):775–784
Pickett JA, Williams IH, Martin AP (1982) (Z)-11-eicosen-1-ol, an important new pheromonal component from the sting of the honey bee, Apis mellifera L. (hymenoptera, Apidae.) J Chem Ecol 8(1):163–175
Robert R, Sokal RR, Rohlf J (1969) Biometry; the principles and practice of statistics in biological research. W. H. Freeman and company, New York
Ruttner F (1988) Biogeography and taxonomy of honeybees. Springer-Verlag, Berlin
Schmidt JO, Morgan ED, Oldham NJ, Do Nascimento RR, Dani FR (1997) Z-11-eicosen-1-ol, a major component of Apis cerana venom. J Chem Ecol 23(8):1929–1939
Shearer DA, Boch R (1965) 2-heptanone in the mandibular gland secretion of the honey bee. Nature 206:530
Southwick EE, Moritz RFA (1987) Effects of meteorological factors on defensive behaviour of honey bees. Int J Biometeorol 31:256–265
Tan K, Hepburn HR, Radloff SE (2005) Heat-balling to Vespa Velutina. Naturwissenschaften 92(10):491–495
Tan K, Radloff SE, Li JJ, Hepburn HR, Yang MX, Zhang LJ, Neumann P (2007) Bee-hawking by the wasp, Vespa Velutina, on the honeybees Apis cerana and A. mellifera. Naturwissenschaften 94(6):469–472
Tan K, Yang MX, Wang ZW, Li H, Zhang ZY, Radloff SE, Hepburn R (2011) Cooperative wasp-killing by mixed-species colonies of honeybees, Apis cerana and Apis mellifera. Apidologie 43(2):195–200
Thom C, Gilley DC, Hooper J, Esch HE (2007) The scent of the waggle dance. PloS Biol 5(10):e228
Ugajin A, Kiya T, Kunieda T, Ono M, Yoshida T, Kubo T (2012) Detection of neural activity in the brains of Japanese honeybee workers during the formation of a "hot defensive bee ball". PLoS One 7(3):e32902
Urlacher E, Frances B, Giurfa M, Devaud JM (2010) An alarm pheromone modulates appetitive olfactory learning in the honeybee (Apis mellifera). Front Behav Neurosci 157(4):1–10
Wager BR, Breed MD (2000) Does honey bee sting alarm pheromone give orientation information to defensive bees? Ann Entomol Soc Am 93(2):1329–1332
Wang ZW, Wen P, Qu YF, Dong SH, Li JJ, Ken T, James CN (2016) Bees eavesdrop upon informative and persistent signal compounds in alarm pheromones. Sci Rep 6:2693
Wittmann D, Radtke R, Zeil J, Lübke G, Francke W (1990) Robber bees (Lestrimelitta limao) and their host chemical and visual cues in nest defense by Trigona (Tetragonisca) angustula (Apidae: Meliponinae). J Chem Ecol 16(2):631–641
Zakour MK, Bienefeld K (2013) Subjective evaluation of defensive behavior in the Syrian honeybee (Apis mellifera syrica). J Apic Sci 57(2):137–145
Acknowledgements
We wish to thank the James Nieh and Richard Corlett whose comments have improved our manuscript. This work was supported by the Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Sericultural & Apicultural Research Institute, Yunnan Academy of Agricultural Sciences. China National Research Fund (31260585) to Ken Tan.
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Zhiwen Gong, Chao Wang and Shihao Dong contributed equally to this work
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Gong, Z., Wang, C., Dong, S. et al. High Concentrations of the Alarm Pheromone Component, Isopentyl Acetate, Reduces Foraging and Dancing in Apis mellifera Ligustica and Apis cerana Cerana. J Insect Behav 30, 188–198 (2017). https://doi.org/10.1007/s10905-017-9606-4
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DOI: https://doi.org/10.1007/s10905-017-9606-4