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Orientation Behavior, Development and Survival of Trichoplusia ni (Lepidoptera: Noctuidae) Larvae on Cotton Expressing Cry1Ac and Cry2Ab and Conventional Cotton

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Abstract

This study was conducted to determine the effects of Bt cotton leaves (Bollgard II), non-Bt cotton leaves, and a mixture of Bt+non-Bt cotton leaves on larval orientation behavior, survival and development of Trichoplusia ni in the laboratory. Results indicate that in a no-choice test, more first and fifth instars remained on Bt leaves than the third instars. All larvae that remained on the leaves gradually moved to leaf edge. In the choice between a Bt and a non-Bt leaf, more first instars moved to non-Bt leaves, whereas the third and fifth instars did not show significant difference in the first 8 h, but eventually more moved to non-Bt leaves. More first instars fed non-Bt leaves than third instars and fifth instars. When larvae fed Bt leaves, 100% of first instars, 92.7% of third instars and 51.1% of fifth instars died in 108 h. Once larvae pupated, >90% developed to adults. First and third instars that fed Bt leaves developed slower but their pupae developed faster than those on Bt+non-Bt leaves, whereas fifth instars developed similar on the three types of leaves. First and third instars that fed Bt+non-Bt leaves resulted in less heavy pupae than those fed non-Bt leaves; whereas the fifth instars that survived on Bt leaves produced lighter pupae.

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Acknowledgements

We thank J. Martinez, Yongmei Zhang, J. Alejandro, J. Caballero, P. Carreon, R. Dominguez, and L. Leal for technical assistance. This article was submitted for publication with the approval of the Center Director of Texas Agricultural Research and Extension Center, Texas A&M University System at Weslaco, Texas, and the Head of the Department of Entomology, Texas A&M University, College Station, Texas.

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  1. Vegetable IPM Laboratory, Department of Entomology, Texas Agricultural Experiment Station, Texas A&M University System, 2415 E. Highway 83, Weslaco, TX, 78596-8399, USA

    Y.-X. Li & T.-X. Liu

  2. Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing, Jiangsu, People’s Republic of China

    Y.-X. Li

  3. PMRU, ARS, USDA, 2413 E. Highway 83, Weslaco, TX, 78596, USA

    S. M. Greenberg

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  1. Y.-X. Li
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Correspondence to T.-X. Liu.

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Li, YX., Greenberg, S.M. & Liu, TX. Orientation Behavior, Development and Survival of Trichoplusia ni (Lepidoptera: Noctuidae) Larvae on Cotton Expressing Cry1Ac and Cry2Ab and Conventional Cotton. J Insect Behav 20, 473–488 (2007). https://doi.org/10.1007/s10905-007-9093-0

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