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A Highly Selective and Sensitive Peptide-Based Fluorescent Ratio Sensor for Ag+

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Abstract

A fluorescence ratio sensor based on dansyl-peptide, Dansyl-Glu-Cys-Glu-Glu-Trp-NH2 (D-P5), was efficiently synthesized by Fmoc solid phase peptide synthesis. The sensor exhibits high selectivity and sensitivity for Ag+ over 16 metal ions in 100 mM sodium perchlorate and 50 mM 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid buffer solution by fluorescence resonance energy transfer. The 1:1 binding stoichiometry of the sensor and Ag+ is measured by fluorescence ratio response and the job’s plot. The dissociation constant of the sensor with Ag+ was calculated to be 6.4 × 10−9 M, which indicates that the sensor has an effective binding affinity for Ag+. In addition, the limit of detection of the sensor for Ag+ was determined to be 80 nM, which also indicates that the sensor has a high sensitivity to Ag+. Result showed that the sensor is an excellent Ag+ sensor under neutral condition. Furthermore, this sensor displays good practicality for Ag+ detection in river water samples without performing tedious sample pretreatment, as well as for silver chloride detection.

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The data used to support the findings of this study are included within the article.

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Funding

This work was supported by the Scientific Research Foundation of Liaocheng University, China (No. 318011919), and the National Natural Science Foundation of China (No. 21974068).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, 252059, People’s Republic of China

    Shuaibing Yu, Zhaolu Wang, Bo Zhang, Lei Wang & Lianzhi Li

  2. Zhong Yuan Academy of Biological Medicine, Liaocheng People’s Hospital Affiliated to Shandong University, Liaocheng, 252000, People’s Republic of China

    Lei Gao

  3. School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094, People’s Republic of China

    Jinming Kong

Authors
  1. Shuaibing Yu
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  2. Zhaolu Wang
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  3. Lei Gao
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  4. Bo Zhang
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  5. Lei Wang
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  6. Jinming Kong
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  7. Lianzhi Li
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Contributions

Shuaibing Yu and Zhaolu Wang contributed equally. Methodology, Conceptualization, Investigation, Writing - original draft. Lei Gao: Investigation, Writing - original draft. Bo Zhang: Investigation. Lei Wang: Visualization, Project administration. Jinming Kong: Methodology, Writing - review & editing. Lianzhi Li: Resources, Supervision, Funding acquisition, Writing - review & editing.

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Correspondence to Lianzhi Li.

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Yu, S., Wang, Z., Gao, L. et al. A Highly Selective and Sensitive Peptide-Based Fluorescent Ratio Sensor for Ag+. J Fluoresc 31, 237–246 (2021). https://doi.org/10.1007/s10895-020-02653-5

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