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Developing an Analytical Method Based on Graphene Quantum Dots for Quantification of Deferiprone in Plasma

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Abstract

In the world of nanotechnology, graphene quantum dots (GQDs) have been considerably employed in numerous optical sensing and bioanalytical applications. Herein, a simple and cost-efficient methodology was developed to the quantification of deferiprone in plasma samples by utilizing the selective interaction of the GQDs and drug in the presence of Fe3+ ions. GQDs were synthesized by a bottom-up technique as an advantageous fluorescent probe. Increasing levels of deferiprone ranging from 5 to 50 mg.L−1, leads to significant fluorescence quenching of GQDs. In addition, the calibration curve was revealed a linear response in this range with a sensitivity of 5 mg.L−1. The method validation was carried out according to the FDA guidelines to confirm the accuracy, precision, stability and selectivity of the developed method. The results show that this green and low-cost fluorescent probe could be used for the analysis of deferiprone.

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Acknowledgments

This work is a part of R.K’s MSc thesis (No. 21) submitted at Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran and supported by Vice Chancellor of for Research of Tabriz University of Medical Sciences, Tabriz, Iran (code: 61288).

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Authors and Affiliations

  1. Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Raha Kaviani

  2. Student Research Committee, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

    Raha Kaviani

  3. Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Saba Ghaffary

  4. Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Abolghasem Jouyban & Ali Shayanfar

  5. Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

    Abolghasem Jouyban & Ali Shayanfar

  6. Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran

    Abolghasem Jouyban

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  1. Raha Kaviani
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  2. Saba Ghaffary
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  3. Abolghasem Jouyban
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  4. Ali Shayanfar
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Correspondence to Ali Shayanfar.

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Kaviani, R., Ghaffary, S., Jouyban, A. et al. Developing an Analytical Method Based on Graphene Quantum Dots for Quantification of Deferiprone in Plasma . J Fluoresc 30, 591–600 (2020). https://doi.org/10.1007/s10895-020-02523-0

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  • DOI: https://doi.org/10.1007/s10895-020-02523-0

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