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Bioimaging of Peroxynitrite in MCF-7 Cells by a New Fluorescent Probe Rhodamine B Phenyl Hydrazide

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Abstract

Peroxynitrite is a potent oxidizing and nitrating agent which has detrimental effects on cells by altering the structure and function of biomolecules present within. A fluorescent probe rhodamine B phenyl hydrazide (RBPH) has been proposed for peroxynitrite (ONOO−) imaging in MCF-7 cells based on its oxidation property, which converts RBPH to pink colored and highly fluorescent rhodamine B. The fluorescence emission intensity of the rhodamine B produced in the above process is linearly related to the concentration of peroxynitrite. The method obeys Beer’s law in the concentration range 2–20 nM and the detection limit has been found to be 1.4 nM. The possible reaction mechanism of peroxynitrite with RBPH to produce rhodamine B has been discussed with spectroscopic evidence. The Probe is selective to the peroxynitrite in the pH range 6–8 which is near physiological pH. Fluorescence microscopic studies suggest that the probe is cell permeable and hence peroxynitrite was imaged in MCF-7 cells.

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Acknowledgments

The authors acknowledge the financial support and award of the fellowship to Gopi A by University grants Commission (UGC), New Delhi, India.

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Authors and Affiliations

  1. Department of Microbiology & Biotechnology, Bangalore University, Jnanabharathi Campus, Bangalore, 560 056, India

    Gopi Ambikapathi, Babu Ramappa Lamani, Devaraju Kuramkote Shivanna, Harish Bodagur Maregowda & Anushree Gupta

  2. Department of Studies in Chemistry, Bangalore University, Central College Campus, Bangalore, 560 001, India

    Suresh Kumar Kempahanumakkagari & Pandurangappa Malingappa

Authors
  1. Gopi Ambikapathi
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  2. Suresh Kumar Kempahanumakkagari
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  3. Babu Ramappa Lamani
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  4. Devaraju Kuramkote Shivanna
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  6. Anushree Gupta
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  7. Pandurangappa Malingappa
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Correspondence to Devaraju Kuramkote Shivanna.

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Ambikapathi, G., Kempahanumakkagari, S.K., Ramappa Lamani, B. et al. Bioimaging of Peroxynitrite in MCF-7 Cells by a New Fluorescent Probe Rhodamine B Phenyl Hydrazide. J Fluoresc 23, 705–712 (2013). https://doi.org/10.1007/s10895-013-1205-y

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  • DOI: https://doi.org/10.1007/s10895-013-1205-y

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