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A Novel Near-Infrared Fluorescent Probe for the Detection of Endogenous Peroxynitrite (ONOO−) in Living Cells

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Abstract

In this study, we present a novel near-infrared (NIR) fluorescent probe Nile-ONO designed for the selective and sensitive detection of ONOO−. The probe Nile-ONO employed Nile red as the fluorophore, with diphenylphosphinate serving as the reaction site. In the presence of ONOO−, the probe Nile-ONO exhibits remarkable fluorescence enhancement at 659 nm, with a response time of less than 20 min and a low detection limit of 0.32 µM. Importantly, MTT assays demonstrate low cytotoxicity in living cells. Furthermore, Nile-ONO has excellent imaging capabilities for endogenous ONOO−. Overall, this work introduces a valuable new method for the rapid detection of ONOO− in biological systems.

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No datasets were generated or analysed during the current study.

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Funding

This work was supported by the Scientific research start-up funds of Jianghan University (No. 0840001), the Scientific Research Project Funding of Jianghan University (No. 2022XKZX27).

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Author notes

  1. Wanxia Gao and Junluan Cai contributed equally to this work.

Authors and Affiliations

  1. Huazhong University of Science and Technology, Wuhan, China

    Jinting Shang, Wanxia Gao, Junluan Cai, Yan Yang, Chen Wang, Na Zhao, Haiping Wang & Yibin Zhang

  2. Advanced Technology Institute of Suzhou Chinese Academy of Science, Co., Ltd, Suzhou, China

    Jinting Shang, Wanxia Gao, Junluan Cai, Yan Yang, Chen Wang, Na Zhao, Haiping Wang & Yibin Zhang

Authors
  1. Jinting Shang
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  2. Wanxia Gao
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  3. Junluan Cai
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  4. Yan Yang
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  5. Chen Wang
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  6. Na Zhao
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Contributions

Wanxia Gao: Writing – review & editing, Writing – original draft, Validation, Methodology, Formal analysis, Conceptualization. Jinting Shang: Methodology, Formal analysis, Conceptualization. Junluan Cai: Writing – review & editing, Methodology, Investigation. Yan Yang: Methodology, Formal analysis, Conceptualization. Chen Wang: Methodology, Investigation. Na Zhao: Methodology, Formal analysis. Yibin Zhang: Methodology, Project administration. Haiping Wang: Visualization, Project administration, Funding acquisition. Jinting Shang: Visualization, Project administration, Funding acquisition.

Corresponding author

Correspondence to Yibin Zhang.

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Shang, J., Gao, W., Cai, J. et al. A Novel Near-Infrared Fluorescent Probe for the Detection of Endogenous Peroxynitrite (ONOO−) in Living Cells. J Fluoresc (2024). https://doi.org/10.1007/s10895-024-03733-6

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