Skip to main content
SpringerLink
Log in

Ultrasensitive Study of Gatifloxacin Based on Its Enhancing Effect on the Cerium (IV)-Sodium Hyposulfite Chemiluminescence Reaction in a Micellar Medium

  • Original Paper
  • Published:
Journal of Fluorescence Aims and scope Submit manuscript

Abstract

A sensitive and rapid flow-injection chemiluminescence (CL) method has been developed for the determination of gatifloxacin in pharmaceutical preparations and biological samples. The method is based on the enhancing effect of gatifloxacin on CL emission generated by the interaction of Ce (IV) in sulphuric acid and sodium hyposulphite (Na2S2O4) sensitized by sodium dodecyl benzene sulfonate (SDBS). Strong CL emission was observed when gatifloxacin was injected into the Ce (IV) in sulphuric acid and Na2S2O4 solution incorporated with SDBS in a flow-cell. Several experimental parameters affecting the CL reaction were investigated and optimized systematically. Under the optimum conditions, it was found that the CL intensity is proportional to the concentration of gatifloxacin in the range of 1.12 × 10−11–4.40 × 10−9 g mL−1 with a co-relation coefficient of 0.9994. The limit of detection was found to be 4.87 × 10−12 g mL−1 and the relative standard deviation (RSD, n = 7) was 1.8% for 4 × 10−8 g mL−1 of GFLX. The proposed method offers higher sensitivity, wide linear range and better stability without requiring sophisticated instrumentation. Thus, the proposed method has been successfully applied to the determination of gatifloxacin in pharmaceuticals, serum and human urine.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 5
Fig. 4

Similar content being viewed by others

References

  1. Perry CM, Douglas O, Miriam H, Onrust SV (2002) Gatifloxacin: a review of its use in the management of bacterial infections. Drugs 62(1):169–207

    Article  PubMed  CAS  Google Scholar 

  2. Saravolatz LD, Leggett J (2003) Gatifloxacin, gemifloxacin, and moxifloxacin: the role of 3 newer fluoroquinolones. Clin Infect Dis 37:1210–1215

    Article  PubMed  CAS  Google Scholar 

  3. Grasela DM (2000) Clinical pharmacology of gatifloxacin, a new fluoroquinolone. Clin Infect Dis 31(Supp 2):S51–S58

    Article  PubMed  CAS  Google Scholar 

  4. Wei L, Chao W, Hong J (2009) Determination of gatifloxacin by spectrophotometric method with malachite green. Huaxue Shiji 31:191–193

    Google Scholar 

  5. Kshirsagar SJ, Chopade VV, Dhaneshwar SR (2008) A sensitive first and second derivative spectrophotometric method and validation for determination of gatifloxacin in bulk and pharmaceutical dosage forms. BioScan 3(2):147–150

    CAS  Google Scholar 

  6. Guo C, Dong P, Chu Z, Wang L, Jiang W (2008) Rapid determination of gatifloxacin in biological samples and pharmaceutical products using europium sensitized fluorescence spectrophotometry. Luminescence 23:7–13

    Article  PubMed  Google Scholar 

  7. Darwish IA, Sultan MA, Al-Arfaj HA (2010) Selective kinetic spectrophotometric method for determination of gatifloxacin based on formation of its N-vinyl chlorobenzoquinone derivative. Spectrochim Acta Part A 75(1):334–339

    Article  Google Scholar 

  8. Amin AS, El-Fetouh Gouda AA, El-Sheikh R, Zahran F (2007) Spectrophotometric determination of gatifloxacin in pure form and in pharmaceutical formulation. Spectrochim Acta Part A 67(5):1306–1312

    Article  Google Scholar 

  9. Guo C, Wang L, Hou Z, Jiang W, Sang L (2009) Micelle-enhanced and terbium-sensitized spectrofluorimetric determination of gatifloxacin and its interaction mechanism. Spectrochim Acta Part A 72(4):766–771

    Article  Google Scholar 

  10. Zhu X, Gong A, Yu S (2008) Fluorescence probe enhanced spectrofluorimetric method for the determination of gatifloxacin in pharmaceutical formulations and biological fluids. Spectrochim Acta Part A 69(2):478–482

    Article  Google Scholar 

  11. Ocana JA, Barragan FJ, Callejon M (2005) Spectrofluorimetric and micelle-enhanced spectrofluorimetric determination of gatifloxacin in human urine and serum. J Pharm Biomed Anal 37(2):327–332

    Article  PubMed  CAS  Google Scholar 

  12. Al-Dgither S, Alvi SN, Hammami MM (2006) Development and validation of an HPLC method for the determination of gatifloxacin stability in human plasma. J Pharm Biomed Anal 41(1):251–255

    Article  PubMed  CAS  Google Scholar 

  13. Overholser BR, Kays MB, Sowinski KM (2003) Determination of gatifloxacin in human serum and urine by high-performance liquid chromatography with ultraviolet detection. J Chromatogr B 798(1):167–173

    Article  CAS  Google Scholar 

  14. Cavazos MLS, Gonzalez LYC, Lerma GG, Torres NW (2006) Determination of gatifloxacin in semen by HPLC with diode-array and fluorescence detection. Chromatographia 63(11–12):605–608

    Article  CAS  Google Scholar 

  15. Nguyen HA, Grellet J, Ba BB, Quentin C, Saux MC (2004) Simultaneous determination of levofloxacin, gatifloxacin and moxifloxacin in serum by liquid chromatography with column switching. J Chromatogr B 810(1):77–83

    CAS  Google Scholar 

  16. Suhagia BN, Shah SA, Rathod IS, Patel HM, Shah DR, Marolia BP (2006) Determination of gatifloxacin and ornidazole in tablet dosage forms by high-performance thin-layer chromatography. Anal Sci 22(5):743–745

    Article  PubMed  CAS  Google Scholar 

  17. Razek TMA, El-Baqary RI, Ramadan AE (2008) Fluorimetric determination of gatifloxacin in aqueous, pure and pharmaceutical formulations. Anal Lett 41(3):417–423

    Article  CAS  Google Scholar 

  18. Bin Z, Shou-jun J, Chang-qin HU (2002) Determination of gatifloxacin by high performance capillary electrophoresis. Chin J Pharm Anal 22:397–400

    Google Scholar 

  19. Ding K, Zhao C, Cao Z, Liu Z, Liu J, Zhan J, Ma C, Xi R (2009) Chemiluminescent detection of gatifloxacin residue in milk. Anal Lett 42(3):505–518

    Article  CAS  Google Scholar 

  20. Xie X, Shao X, Yue Q, Huang C, Song Z (2007) Ultrasensitive assay of gatifloxacin at picogram level based on its enhancing effect on the myoglobin-luminol chemiluminescence reaction. Anal Lett 40(10):1951–1961

    Article  CAS  Google Scholar 

  21. Sun H, Li L, Chen X (2008) Determination of gatifloxacin in injection solutions and urine by europium-sensitized chemiluminescence. Asian J Pharm Sci 3(4):168–174

    Google Scholar 

  22. Mestre YF, Zamora LL, Calatayud JM (2001) Flow-chemiluminescence: a growing modality of pharmaceutical analysis. Luminescence 16(3):213–235

    Article  PubMed  CAS  Google Scholar 

  23. Townshend A, Ruengsitagoon W, Thongpoon C, Liawruangrath S (2005) Flow injection chemiluminescence determination of tetracycline. Anal Chim Acta 541(1–2):105–111

    CAS  Google Scholar 

  24. Anastos N, Barnet NW, Lewis SW, Gathergood N, Scammells PJ, Sims DN (2005) Determination of psilocin and psilocybin using flow injection analysis with acidic potassium permanganate and tris(2, 2′-bipyridyl)ruthenium(II) chemiluminescence detection respectively. Talanta 67(2):354–359

    Article  PubMed  CAS  Google Scholar 

  25. Liang YD, Song JF, Tian T (2004) Determination of pipemidic acid based on flow-injection chemiluminescence due to energy transfer from peroxynitrous acid synthesized on-line. Anal Bioanal Chem 380(7–8):918–923

    Article  PubMed  CAS  Google Scholar 

  26. Wolkoff AW, Larose RH (1975) Separation and detection of low concentrations of polythionates by high speed anion exchange liquid chromatography. Anal Chem 47(7):1003–1008

    Article  CAS  Google Scholar 

  27. Kenner CT, Bush KW (1979) Quantitative Analysis. Macmillan, New York, p 375

    Google Scholar 

Download references

Acknowledgements

This research was supported by Kyungpook National University Research Fund, 2009.

Author information

Authors and Affiliations

  1. Department of Chemistry, Kyungpook National University, Daegu, 702-701, South Korea

    Mohammad Kamruzzaman, Al-Mahmnur Alam, Taslima Ferdous & Sang Hak Lee

  2. Research Institute of Advanced Energy Technology, Kyungpook National University, Daegu, 702-701, South Korea

    Young Ho Kim

  3. Korea Basic Science Institute Daegu Center, Daegu, 702-701, South Korea

    Sang Hak Lee & Sung Hong Kim

Authors
  1. Mohammad Kamruzzaman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Al-Mahmnur Alam
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Taslima Ferdous
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sang Hak Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Young Ho Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Sung Hong Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sang Hak Lee.

Electronic supplementary material

Below is the link to the electronic supplementary material.

ESM 1

(DOC 180 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kamruzzaman, M., Alam, AM., Ferdous, T. et al. Ultrasensitive Study of Gatifloxacin Based on Its Enhancing Effect on the Cerium (IV)-Sodium Hyposulfite Chemiluminescence Reaction in a Micellar Medium. J Fluoresc 21, 1539–1545 (2011). https://doi.org/10.1007/s10895-011-0842-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10895-011-0842-2

Keywords

Search

Navigation