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Flow-injection chemiluminescence method for the determination of moxifloxacin in pharmaceutical tablets and human urine using silver nanoparticles sensitized calcein–KMnO4 system

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Abstract

Silver nanoparticles have been synthesized and were utilized for the enhanced luminometric estimation of moxifloxacin antibiotic. During the experimental procedure, it was clearly found that the addition of silver nanoparticles intensifies the weak chemiluminescence signal intensity of calcein–KMnO4 system by several folds. It was also obvious that the intensity enhancement was linearly proportional to the moxifloxacin concentration and this phenomenon was further utilized for the quantitative determination of target analyte. Effects of the different chemical variables during the experiment were studied to achieve best chemiluminescence signal. Under the optimized experimental parameters, the linear calibration graph was established over the moxifloxacin concentration range of 6.0 × 10−8 M to 2.5 × 10−6 M with coefficient of correlation (r 2) value 0.9998. The lower detection limit was found to be 5.6 × 10−9 M. The percentage relative standard deviation calculated from five replicate chemiluminescence measurements was found to be 2.63 %. The developed chemiluminescence technique was successfully applied to the determination of moxifloxacin in tablet formulation and spiked human urine sample.

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Acknowledgments

The authors would like to extend their gratitude to the Deanship of Scientific Research, College of Science Research Center, King Saud University, Riyadh, Saudi Arabia for supporting this project.

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Authors and Affiliations

  1. Advanced Materials Research Chair, Department of Chemistry, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia

    Saikh Mohammad Wabaidur, Zeid A. Alothman, Masoom Raza Siddiqui, Mu Naushad & Ayoub A. Alqadami

  2. Department of Chemistry, Aliah University, Kolkata, West Bengal, India

    Seikh Mafiz Alam

Authors
  1. Saikh Mohammad Wabaidur
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  2. Seikh Mafiz Alam
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  3. Zeid A. Alothman
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  4. Masoom Raza Siddiqui
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  5. Mu Naushad
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  6. Ayoub A. Alqadami
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Correspondence to Saikh Mohammad Wabaidur or Seikh Mafiz Alam.

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Wabaidur, S.M., Alam, S.M., Alothman, Z.A. et al. Flow-injection chemiluminescence method for the determination of moxifloxacin in pharmaceutical tablets and human urine using silver nanoparticles sensitized calcein–KMnO4 system. Bioprocess Biosyst Eng 38, 1803–1810 (2015). https://doi.org/10.1007/s00449-015-1433-4

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  • DOI: https://doi.org/10.1007/s00449-015-1433-4

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